高等学校化学学报

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (5): 860.doi: 10.7503/cjcu20170120

• 高分子化学 • 上一篇    下一篇

载药荧光纳米粒子的制备及在乳腺癌MCF-7细胞系的应用及效果评价

张海鹏1, 韩冰1, 贾致真3, 丁荣波1, 徐斌2, 徐蔚青2, 范志民1   

  1. 1. 吉林大学第一医院, 长春 130021;
    2. 吉林大学超分子结构与材料国家重点实验室, 长春 130012;
    3. 东北师范大学计算机科学与信息技术学院, 长春 130117
  • 收稿日期:2017-02-27 修回日期:2017-04-18 出版日期:2017-05-10 发布日期:2017-04-18
  • 通讯作者: 范志民,男,博士,教授,博士生导师,主要从事乳腺癌诊断与治疗相关研究.E-mail:fanzhimn@163.com E-mail:fanzhimn@163.com
  • 基金资助:

    吉林省科技厅自然科学基金项目（批准号：20150101205JC）、吉林大学白求恩医学科研支持计划项目（批准号：2013205023）和吉林大学第一医院院青年基金（批准号：JDYY72016040）资助.

Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell

ZHANG Haipeng1, HAN Bing1, JIA Zhizhen3, DING Rongbo1, XU Bin2, XU Weiqing2, FAN Zhimin1   

  1. 1. The First Hospital of Jilin University, Changchun 130021, China;
    2. State Key Laboratory for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China;
    3. College of Computer Science and Information Technology, Northeast Normal University, Changchun 130117, China
  • Received:2017-02-27 Revised:2017-04-18 Online:2017-05-10 Published:2017-04-18
  • Supported by:

    Supported by the Natural Science Foundation of Jilin Province Science and Technology Department, China(No.20150101205JC), the Jilin University Bethune Scientific Research Support Plan, China(No.2013205023) and the Youth Foundation of the First Hosptial of Jilin University, China(No.JDYY72016040).

RichHTML

PDF (PC)

被引次数

1

摘要：

利用两亲性聚乙二醇-聚乳酸共聚物（PEG-PDLLA）包覆荧光染料（DPBA）和紫杉醇（PTX），通过自组装方法制得载药荧光纳米粒子DPBA/PTX@PEG-PDLLA.纳米粒子尺寸均一，具有良好的生物相容性.对纳米粒子的发光性质、载药量和体外药物释放等进行了表征，并考察了纳米粒子对乳腺癌细胞MCF-7的抑制效果，观察了MCF-7细胞对纳米粒子的摄取情况.结果表明，DPBA/PTX@PEG-PDLLA纳米粒子具有较强的红光发射，不仅可以用于MCF-7肿瘤细胞质荧光成像，而且对肿瘤细胞的增殖具有一定的抑制能力.

关键词: 载药荧光纳米粒子, 自组装, 药物释放, 生物成像, 紫杉醇

Abstract:

Drug-loaded fluorescent nanoparticles(DPBA/PTX@PEG-PDLLA) were prepared by amphiphilic polymers(PEG-PDLLA) coated with AIE dyes(DPBA) and paclitaxel(PTX). The effects of capacity of DPBA and PTX on the photophysical properties of nanoparticles were investigated. In addition, the drug release in vitro of nanoparticles as well as the inhibitory effect of nanoparticles on breast cancer MCF-7 cells were investigated. Moreover, the nanoparticle uptake of MCF-7 cells was observed. As conclusion, the DPBA/PTX@PEG-PDLLA nanoparticles exhibit strong red emission(654 nm) with high fluorescence quantum yield up to 25%. The nanoparticles have uniform size and good biocompatibility. The freshly prepared nanoparticles have pretty drug release ability, and the cumulative release rate of 48 h can reach 25.1%. The results of in vitro MTT and CLSM experiments show that DPBA/PTX@PEG-PDLLA nanoparticles not only have good inhibiting ability with the cell proliferation of MCF-7 tumor cells, but also can be uptake by tumor cell and then absorbed by their cytoplasmic fluorescence imaging.

Key words: Drug loaded fluorescent nanoparticles, Self-assembly, Drug release, Bioimaging, Paclitaxel

中图分类号: 

TrendMD: