高等学校化学学报

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (4): 691.doi:

• 研究论文 • 上一篇    下一篇

壳聚糖包裹硅胶载体印迹牛血红蛋白的研究

刘秋叶1, 何锡文1, 李文友1, 陈朗星1, 张玉奎1,2   

    1. 南开大学化学系, 天津 300071;
    2. 中国科学院大连化学物理研究所, 大连 116011
  • 收稿日期:2008-07-07 出版日期:2009-04-10 发布日期:2009-04-10
  • 通讯作者: 何锡文, E-mail:xiwenhe@nankai.edu.cn; 张玉奎, E-mail:ykzhang@dicp.ac.cn
  • 基金资助:

    国家“九七三”计划(批准号: 2007CB914100)和国家自然科学基金(批准号: 20675040, 20875049, 20875050)资助.

Studies on Chitosan Coated on Silica for Imprinting Bovine Hemoglobin

LIU Qiu-Ye1, HE Xi-Wen1*, LI Wen-You1, CHEN Lang-Xing1, ZHANG Yu-Kui1,2*   

    1. Department of Chemistry, Nankai University, Tianjin 300071, China;
    2. Dalian Institution of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China
  • Received:2008-07-07 Online:2009-04-10 Published:2009-04-10
  • Contact: HE Xi-Wen, E-mail:xiwenhe@nankai.edu.cn; ZHANG Yu-Kui, E-mail:ykzhang@dicp.ac.cn

PDF (PC)

被引次数

32

摘要：

用壳聚糖包裹的硅胶为载体, 利用壳聚糖表面的氨基与戊二醛结合, 在硅胶表面引入醛基, 固定模板蛋白(牛血红蛋白). 用溶胶-凝胶过程再次包裹固定蛋白质的载体, 洗去模板蛋白后, 得到具有选择性识别的牛血红蛋白分子印迹聚合物. 用红外光谱(IR)、扫描电子显微镜(SEM)和元素分析对聚合物进行了表征, 结果表明, 载体表面成功地接枝了分子印迹聚合物. 选择性吸附实验结果表明, 分子印迹聚合物具有良好的识别性能, 能实现水溶液中牛血红蛋白的富集.

关键词: 分子印迹聚合物, 牛血红蛋白, 硅胶, 壳聚糖

Abstract:

The silica modified with chitosan was synthesized as a carrier material. The glutaraldehyde was coupled with amido group of chitosan, and template protein-bovine hemoglobin was immobilized by aldehyde group. Then, the carrier was coated by sol-gel, after template protein was removed; molecularly imprinted polymer was prepared for selectively recognizing bovine hemoglobin(BHb). The results of IR and scanning electron microscope(SEM) demonstrated the formation of a grafting polymer layer on the modified silica gel surface. Selectivity adsorption experiments under equilibrium adsorption show that the imprinted polymers have good adsorption capacity and specific recognition for BHb in aqueous solution, and imprinted polymer hold good recognition capability for BHb when completive substances are present. The MIP synthesis strategy is promising for separating and purifying specific protein compositions in bio-samples. It may have good prospect for riching target protein, which is required to further study.

Key words: Molecular imprinted polymer, Bovine hemoglobin, Silica gel, Chitosan

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