高等学校化学学报 ›› 2020, Vol. 41 ›› Issue (9): 2099-2106.doi: 10.7503/cjcu20200217

• 高分子化学 • 上一篇    

氧化石墨烯-海藻酸钠-壳聚糖复合支架的制备及表征

王博蔚1, 马瑞2, 吴凡1, 刘志辉2, 李凌锋2, 张骁2, 刘定坤2, 杨楠2, 李美慧2, 杨德峰1(), 孙琪2   

  1. 1.吉林大学第二医院, 长春 130021
    2.吉林大学口腔医院, 长春 130041
  • 收稿日期:2020-04-20 出版日期:2020-09-10 发布日期:2020-09-02
  • 通讯作者: 杨德峰 E-mail:yangdefeng1979@sina.com
  • 基金资助:
    吉林省科技厅重点科技研发项目(20180201056YY);吉林省科技厅医药健康项目(20190304032YY)

Preparation and Characterization of Graphene Oxide-sodium Alginate-chitosan Composite Scaffold

WANG Bowei1, MA Rui2, WU Fan1, LIU Zhihui2, LI Lingfeng2, ZHANG Xiao2, LIU Dingkun2, YANG Nan2, LI Meihui2, YANG Defeng1(), SUN Qi2   

  1. 1.The Second Hospital of Jilin University, Changchun 130021, China
    2.Stomatology Hospital of Jilin University, Changchun 130041, China
  • Received:2020-04-20 Online:2020-09-10 Published:2020-09-02
  • Contact: YANG Defeng E-mail:yangdefeng1979@sina.com
  • Supported by:
    Supported by the Key Science and Technology Research and Development Project of Jilin Provincial Department of Science and Technology, China(20180201056YY);Medicine and Health Project of Jilin Provincial Science and Technology Department, China(20190304032YY)

摘要:

通过冷冻干燥技术, 将不同量的氧化石墨烯与海藻酸钠和壳聚糖复合, 构建复合支架材料. 研究了不同的氧化石墨烯含量(质量分数0, 0.3%, 0.5%, 0.7%, 1%)对支架材料微观结构、 孔隙率、 溶胀比、 体外降解性能、 机械性能及生物相容性的影响, 以确定复合支架中最佳氧化石墨烯含量. 研究结果表明, 复合材料呈固态海绵状结构, 具有一定的形态可塑性; 扫描电子显微镜观察发现, 各组支架均为三维网状结构, 随着氧化石墨烯含量的增加, 孔隙尺寸逐渐降低, 孔壁厚度增加, 孔隙尺寸在140~240 μm之间; 随氧化石墨烯含量的增加, 复合支架溶胀比和体外降解速率逐渐降低, 而机械强度明显增强; 体外细胞毒性显示, 当氧化石墨烯质量分数为0.3%时, 细胞存活率最高, 而当氧化石墨烯含量增高时, 细胞活性会被明显抑制, 造成细胞死亡. 因此, 氧化石墨烯在复合支架中最佳含量为0.3%.

关键词: 支架, 氧化石墨烯, 海藻酸钠, 壳聚糖

Abstract:

The composite scaffold material was constructed by freeze-drying technology from graphene oxide, sodium alginate and chitosan. The effects of graphene oxide content on the microstructure, porosity, swelling ratio, in vitro degradation performance, mechanical properties and biocompatibility of the scaffold materials were studied to determine the optimal graphene oxide content in the composite scaffold. The results show that the composite material has a solid sponge-like structure with a certain morphological plasticity. Scanning electron microscopy(SEM) observations show that each group of supports has a three-dimensional network structure. As the content of graphene oxide increased, the pore size gradually decreased and the pore wall thickness increased, the swelling ratio and degradation rate of the composite scaffold gradually decreased while the mechanical strength was significantly enhanced. Results of in vitro cytotoxicity showed that when the graphene oxide content was 0.3%(mass fraction), the cell survival rate was the highest, and when the graphene oxide content increased, the cell activity was significantly inhibited, causing cell death. Finally, the optimal content of graphene oxide in the composite scaffold is 0.3%. Our research lays a good foundation for the application of graphene oxide in bone tissue engineering materials.

Key words: Scaffold, Graphene oxide, Sodium alginate, Chitosan

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