基于镍丝负载氧化镍纳米片的尿酸生物传感器

doi:10.7503/cjcu20180519

高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (2): 240.doi: 10.7503/cjcu20180519

基于镍丝负载氧化镍纳米片的尿酸生物传感器

贾宏亮, 赵建伟(), 秦丽溶, 赵敏

西南大学物理科学与技术学院, 重庆 400715

收稿日期:2018-07-24 出版日期:2019-02-10 发布日期:2018-11-16
作者简介:
联系人简介: 赵建伟, 男, 博士, 副教授, 主要从事纳米材料合成及电化学性能方面的研究. E-mail: zhaojw@swu.edu.cn
基金资助:
国家自然科学基金(批准号: 11204246)和中央高校基本科研业务费专项资金(批准号: XDJK2018B033)资助

Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets^†

JIA Hongliang, ZHAO Jianwei^*(), QIN Lirong, ZHAO Min

School of Physical Science and Technology, Southwest University, Chongqing 400715, China

Received:2018-07-24 Online:2019-02-10 Published:2018-11-16
Contact: ZHAO Jianwei E-mail:zhaojw@swu.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.11204246) and the Fundamental Research Funds for the Central Universities, China(No.XDJK2018B033)

摘要/Abstract

摘要：

利用水热法与高温热处理相结合, 在金属镍丝表面制备了氧化镍纳米片, 这些纳米片相互交错, 均匀覆盖于镍丝表面, 其宽度约为数百纳米, 厚度约10 nm. 将镍丝负载氧化镍纳米片吸附固定尿酸氧化酶后, 得到尿酸生物传感器电极, 该电极显示出优异的电化学性能, 其灵敏度达到821.4 μA/(mmol·cm²), 线性检测范围为1~900 μmol/L, 检出限为0.1 μmol/L, 同时具有良好的抗干扰特性. 该尿酸传感器电极易于进行植入式探测或与微流控技术相结合, 为快速、高灵敏的尿酸检测提供了新途径.

关键词: 镍丝, 纳米结构, 水热法, 尿酸, 生物传感

Abstract:

Ni wire modified with NiO nanosheets was synthesized by hydro-thermal method accompanied with heat treatment at high-temperature. The characterized results revealed that these NiO nanosheets grew uniformly on the surface of the Ni wire, with a size of several hundreds of nanometers and a thickness of about ten nanometers. Electrochemical experiments showed that the Ni wire modified with NiO nanosheets could be used as a kind of good material for immobilization of uricase to fabricate an efficient uric acid biosensor. Sensitivity of the prepared biosensor was found to be 821.4 μA/(mmol·cm²). The linear range for the detection was 1—900 μmol/L. The detection limit was 0.1 μmol/L. These results indicate that the Ni wire modified with NiO nanosheets is a new platform for the construction of biosensors.

Key words: Ni wire, Nanostructures, Hydrothermal method, Uric Acid, Biosensor

中图分类号:

O657.1

TrendMD:

贾宏亮, 赵建伟, 秦丽溶, 赵敏. 基于镍丝负载氧化镍纳米片的尿酸生物传感器. 高等学校化学学报, 2019, 40(2): 240.

JIA Hongliang,ZHAO Jianwei,QIN Lirong,ZHAO Min. Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets^†. Chem. J. Chinese Universities, 2019, 40(2): 240.

图/表 8

Fig.1 Schematic illustration and photograph of the prepared nafion/uricase/NiO-nanosheets/Ni bioelectrodes

Fig.2 Low magnification(A) and high magnification(B) SEM images of the prepared Ni wire modified with NiO nanosheets

Fig.3 EDS spectrum(A) and TEM image(B) of the prepared NiO nanosheetsInset of (B): corresponding SAED pattern.

Fig.4 CV curves of the prepared electrode in the different concentration of UA(A) and typical CVs of the electrode in 0.01 mol PBS solution containing 0.2 mmol UA at different scan rates(from 50 mV/s to 280 mV/s)(B)Inset of (B): plots of peak currents vs. ν1/2.

Fig.5 Typical current-time response for the prepared electrode(A) or the comparison electrode to successive additions of different concentrations of UA in a stirring PBS solution at an applied potential of 0.3 V vs. Ag/AgCl(B)Inset of (B): the linear relationship between the catalytic current and the concentration of UA.

Table 1 Comparison of different electrode materials for the determination of UA

Type of electrode	Sensitivity/ (μA·mmol^-1·cm^-2)	Linear range/ (μmol·L^-1)	Detection limit/(μmol·L^-1)	Ref.
Uricase/Au NP/c-MWCNT/Au	440.0	10—800	10.0	[18]
Uricase/CeO_2-_x/C/rGO/GCE	284.5	49.8—1050.0	2.0	[19]
Nafion/Uricase/ZnO NSs/Ag/Si	129.81	50—2000	0.019	[14]
Uricase/Ni microdiscs/NiO/ITO	431.09	50—1000	30	[20]
Nafion/Uricase/ZnO/Au	89.74	100—590	25.6	[13]
CoNi nanoparticles/C	248.2	25—575	0.08	[21]
Nanoporous RuO₂/Au	344.2	20—1000	9.6	[22]
Nafion/Uricase/NiO/Ni	821.4	1—900	0.1	This work

Fig.6 Amperometric curve of addition of UA and some potential interference

Table 2 Determination of UA in real urine samples using our biosensor(n=3)

Real urine sample	Concentration of UA detected/ (μmol·L^-1)	Spiked UA concentration/ (μmol·L^-1)	Theoretical total UA concentration/ (μmol·L^-1)	Measured total UA concentration/ (μmol·L^-1)	Recovery(%)
1	235.7	100	335.7	343.6	102.4
2	218.4	200	418.4	402.3	96.2
3	411.2	400	811.2	774.9	95.5

参考文献 22

[1]	Erden P. E., Kilic E., Talanta,2013, 107, 312-323
[2]	Fang B., Feng Y., Wang G., Zhang C., Gu A., Liu M., Microchim. Acta,2011, 173, 27-32
[3]	Ma Q. S., Wang Q., Zhao K. Z., Zhai S. B., Liu S., Liu Z. Q., Chem. J. Chinese Universities,2013, 34(12), 2716-2720
	(马青山, 王茜, 赵凯姝, 翟淑波, 刘舒, 刘志强. 高等学校化学学报, 2013, 34(12), 2716-2720)
[4]	Jin D., Seo M. H., Huy B. T., Pham Q. T., Conte M. L., Thangadurai D., Lee Y. I., Biosens. Bioelectron.,2016, 77, 359-365
[5]	Li X., Li G., Jiang Y., Kang D., Jin C., Shi Q., Jin T., Inoue K., Todoroki K., Toyo’oka T., Min J., J. Chromatogr. B,2015, 1002, 394-398
[6]	Liu B. H., Deng J. Q., Chem. J. Chinese Universities,1996, 17(5), 702-706
	(刘宝红, 邓家祺. 高等学校化学学报, 1996, 17(5), 702-706)
[7]	Dai H., Wang N., Wang D., Zhang X., Ma H., Lin M., Microchim. Acta,2016, 183, 3053-3059
[8]	Hou C., Liu H., Zhang D., Yang C., Zhang M., J. Alloy. Compd.,2016, 666, 178-184
[9]	KanJ. Q., Mu S. L., Acta Phys.-Chim. Sin.,1993, 9(3), 345-350
	(阚锦晴, 穆绍林. 物理化学学报, 1993, 9(3), 345-350)
[10]	Zhao J., Mu F., Qin L., Jia X., Yang C., Mater. Chem. Phys.,2015, 166, 176-181
[11]	Zhao J., Qin L., Hao Y., Guo Q., Mu F., Yan Z., Microchim. Acta,2012, 178, 439-445
[12]	Suresh R., Giribabu K., Manigandan R., Stephenb A., Narayanan V., RSC Adv.,2014, 4, 17146
[13]	Zhao Y., Yan X., Kang Z., Lin P., Fang X., Lei Y., Ma S., Zhang Y., Microchim. Acta,2013, 180, 759-766
[14]	Ahmad R., Tripathy N., Jang N. K., Khang G., Hahn Y., Sensor. Actuat. B,2015, 206, 146-151
[15]	Cheng C., Kao C. Y., Electroanal.,2016, 28, 695-703
[16]	Tian K., Prestgard M., Tiwari A., Mater. Sci. Eng. C,2014, 41, 100-118
[17]	Duan C., Zhao J., Qin L. Yang L., Zhou Y., Mater. Lett.,2017, 208, 65-68
[18]	Chauhan N., Pundir C. S., Anal. Biochem.,2011, 413, 97-103
[19]	Peng B., Cui J., Wang Y., Liu J., Zheng H., Jin L., Zhang X., Zhang Y., Wu Y., Nanoscale, 2018, 10, 1939-1945
[20]	Arora K., Tomar M., Gupta V., Analyst,2014, 139, 4606-4612
[21]	Singh B., Laffir F., Dickinson C., McCormac T., Dempsey E., Electroanal.,2011, 23(1), 79-89
[22]	Yang Y., Jo A., Lee Y., Lee C., Sens. Actuators B,2018, 255, 316-324

基于镍丝负载氧化镍纳米片的尿酸生物传感器

Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets^†

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基于镍丝负载氧化镍纳米片的尿酸生物传感器

Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets†

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Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets^†