高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (2): 240-245.doi: 10.7503/cjcu20180519

• 分析化学 • 上一篇    下一篇

基于镍丝负载氧化镍纳米片的尿酸生物传感器

贾宏亮, 赵建伟(), 秦丽溶, 赵敏   

  1. 西南大学物理科学与技术学院, 重庆 400715
  • 收稿日期:2018-07-24 出版日期:2019-02-10 发布日期:2018-11-16
  • 作者简介:

    联系人简介: 赵建伟, 男, 博士, 副教授, 主要从事纳米材料合成及电化学性能方面的研究. E-mail: zhaojw@swu.edu.cn

  • 基金资助:
    国家自然科学基金(批准号: 11204246)和中央高校基本科研业务费专项资金(批准号: XDJK2018B033)资助

Uric Acid Biosensor Based on Ni Wire Modified with NiO Nanosheets

JIA Hongliang, ZHAO Jianwei*(), QIN Lirong, ZHAO Min   

  1. School of Physical Science and Technology, Southwest University, Chongqing 400715, China
  • Received:2018-07-24 Online:2019-02-10 Published:2018-11-16
  • Contact: ZHAO Jianwei E-mail:zhaojw@swu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(No.11204246) and the Fundamental Research Funds for the Central Universities, China(No.XDJK2018B033)

摘要:

利用水热法与高温热处理相结合, 在金属镍丝表面制备了氧化镍纳米片, 这些纳米片相互交错, 均匀覆盖于镍丝表面, 其宽度约为数百纳米, 厚度约10 nm. 将镍丝负载氧化镍纳米片吸附固定尿酸氧化酶后, 得到尿酸生物传感器电极, 该电极显示出优异的电化学性能, 其灵敏度达到821.4 μA/(mmol·cm2), 线性检测范围为1~900 μmol/L, 检出限为0.1 μmol/L, 同时具有良好的抗干扰特性. 该尿酸传感器电极易于进行植入式探测或与微流控技术相结合, 为快速、 高灵敏的尿酸检测提供了新途径.

关键词: 镍丝, 纳米结构, 水热法, 尿酸, 生物传感

Abstract:

Ni wire modified with NiO nanosheets was synthesized by hydro-thermal method accompanied with heat treatment at high-temperature. The characterized results revealed that these NiO nanosheets grew uniformly on the surface of the Ni wire, with a size of several hundreds of nanometers and a thickness of about ten nanometers. Electrochemical experiments showed that the Ni wire modified with NiO nanosheets could be used as a kind of good material for immobilization of uricase to fabricate an efficient uric acid biosensor. Sensitivity of the prepared biosensor was found to be 821.4 μA/(mmol·cm2). The linear range for the detection was 1—900 μmol/L. The detection limit was 0.1 μmol/L. These results indicate that the Ni wire modified with NiO nanosheets is a new platform for the construction of biosensors.

Key words: Ni wire, Nanostructures, Hydrothermal method, Uric Acid, Biosensor

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