高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (8): 1529.

• 研究论文 • 上一篇    下一篇

小檗碱与人免疫球蛋白在仿生膜中的相互作用

李颖1, 王超1, 胡之德2   

  1. 1. 河海大学环境学院, 浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
    2. 兰州大学化学与化工学院, 兰州 730000
  • 收稿日期:2009-11-04 出版日期:2010-08-10 发布日期:2010-08-10
  • 通讯作者: 李颖, 女, 博士, 讲师, 主要从事生物大分子与小分子相互作用及环境污染物的分析研究. E-mail: hj6688@hhu.edu.cn
  • 基金资助:

    教育部重点实验室开放基金(批准号: 2007KJ002)和中央高校基本科研业务费专项资金(批准号: B1020182)资助.

Interaction Between Berberine and Human Gamma Globulin in Membrane Mimetic Environments

LI Ying1*, WANG Chao1, HU Zhi-De2   

  1. 1. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment,    Hohai University, Nanjing  210098,  China;
    2. College of Chemistry and Chemical Engineering,  Lanzhou University, Lanzhou 730000,  China
  • Received:2009-11-04 Online:2010-08-10 Published:2010-08-10
  • Contact: LI Ying. E-mail: hj6688@hhu.edu.cn
  • Supported by:

    教育部重点实验室开放基金(批准号: 2007KJ002)和中央高校基本科研业务费专项资金(批准号: B1020182)资助.

摘要: 利用荧光光谱、圆二色谱(CD)和动态激光散射技术研究了小檗碱与人免疫球蛋白(HGG)在以双(2-乙基己酯)-磺酸基琥珀酸钠(AOT)/异辛烷/水微乳液为膜的模拟环境下的结合反应. 通过Scatchard方程分别计算了不同温度下的反应结合常数. CD光谱结果表明: 小檗碱与HGG键合改变了蛋白原有的构象. 同时由热力学结果可知, 小檗碱与HGG之间的相互作用主要以疏水作用为主, 同时存在静电作用力和氢键作用力. 动态激光散射结果进一步证明了药物与HGG在微乳液中的相互作用. 通过计算机模拟技术, 在不考虑环境影响的条件下从理论上探讨了HGG与小檗碱的结合区域和结合模式.

关键词: 小檗碱, 人免疫球蛋白, 相互作用

Abstract: The interaction between berberine and human gamma globulin(HGG) in AOT/ isoctant/water reverse micellar was studied by fluorescence quenching technique, UV absorption spectroscopy, circular dischroism(CD) spectroscopy and dynamic light scattering(DLS) technique. The interaction of HGG with berberine at 289, 296, 303 and 310 K in water-surfactant molar ratio(ω0=25) reverse micellar was characterized by one binding site with the affinity constant K at 4.50×104, 4.18×104, 4.13×104 and 3.76×104 L/mol, respectively. The affinities in reverse micellar were much higher than that in buffer solution. The CD spectra results proved that the protein secondary structure changed in the reverse micellar in the absence and presence of berberine compared with the free form of HGG in buffer. The binding process was exothermic and spontaneous, as indicated by the thermodynamic analyses. The results show that hydrophobic and electrostatic interaction play the main roles in the binding of berberine to HGG. Furthermore, the DLS data suggested that HGG may locate inside of the reverse micellar and berberine could interact with them.

Key words: Berberine, Human gamma globulin, Interaction

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