高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (12): 2366.

• 研究论文 • 上一篇    下一篇

核酸适体修饰纳米金-钌催化共振散射光谱法测定痕量Pb2+

梁爱惠, 张静, 温桂清, 刘庆业, 李廷盛, 蒋治良   

  1. 广西师范大学环境与资源学院, 珍稀濒危动植物生态与环境保护省部共建教育部重点实验室, 桂林 541004
  • 收稿日期:2010-05-06 修回日期:2010-05-24 出版日期:2010-12-10 发布日期:2010-12-06
  • 通讯作者: 蒋治良 E-mail:zljiang@mailbox.gxnu.edu.cn
  • 作者简介:蒋治良, 男, 博士, 教授, 博士生导师, 从事纳米材料化学与光谱分析研究. E-mail: zljiang@mailbox.gxnu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号: 21075023, 20865002, 20965002)、 广西省自然科学基金(批准号: 0991021z)和珍稀濒危动植物生态与环境保护省部共建教育部重点实验室项目资助.

Aptamer-modified AuRu Nanoparticle Catalytic Resonance Scattering Spectral Assay for the Determination of Trace Pb2+

LIANG Ai-Hui, ZHANG Jing, WEN Gui-Qing, LIU Qing-Ye, LI Ting-Sheng, JIANG Zhi-Liang*   

  1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource Sciences, Guangxi Normal University, Guilin 541004, China
  • Received:2010-05-06 Revised:2010-05-24 Online:2010-12-10 Published:2010-12-06
  • Contact: JIANG Zhi-Liang E-mail:zljiang@mailbox.gxnu.edu.cn
  • About author:蒋治良, 男, 博士, 教授, 博士生导师, 从事纳米材料化学与光谱分析研究. E-mail: zljiang@mailbox.gxnu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 21075023, 20865002, 20965002)、 广西省自然科学基金(批准号: 0991021z)和珍稀濒危动植物生态与环境保护省部共建教育部重点实验室项目资助.

摘要: 用铅离子的特异性核酸适体(aptamer)修饰AuRu复合纳米微粒(AuRu的摩尔比为5:1)制备了铅离子的核酸适体纳米探针(AptAuRu).在pH 7.0的Na2HPO4-NaH2PO4缓冲溶液及85 mmol/L NaCl存在下,AptAuRu纳米探针亦不聚集. 当Pb2+ 存在时,Pb2+可与探针中的aptamer形成较稳定的G-四分体结构,从而析放出AuRu复合纳米微粒并进一步聚集形成较大的微粒,导致592 nm处的共振散射光强度线性增大. 该反应液经0.15 μm滤膜过滤后,获得未反应的AptAuRu滤液. 滤液中的纳米微粒对氯酸钠-碘化钠反应具有较强的催化作用,其产物与阳离子表面活性剂形成缔合微粒,在472 nm处有一较强的共振散射峰. 随着Pb2+浓度增大,滤液中金钌纳米微粒浓度降低,其催化作用减弱,共振散射强度值降低. Pb2+浓度在0.12~60 pM范围与其共振散射强度降低值ΔI472nm呈线性关系,回归方程、相关系数分别为ΔI472nm =3.1C+7.3,0.9967, 检出限为0.08 pM Pb2+. 将本法用于废水中Pb2+的检测,其结果令人满意.

关键词: Pb2+, 核酸适体, 纳米金钌, 催化共振散射光谱法

Abstract: The Au/Ru nanoparticle, in the mole ratio of 5/1, was modified by aptamer to prepare an aptamer AuRu nanoprobe (AptAuRu) for Pb2+, and the AptAuRu probe was stable in pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of 85 mmol/L NaCl. Upon addition of Pb2+, the nanoprobe reacted with Pb2+ to form very stable G-quadruplex, and the released AuRu nanoparticles aggregated to big particles, which result to the resonance scattering (RS) peak at 592 nm increasing. The increased RS intensity was linear to the concentration of Pb2+ in the range of 1.2-240 pmol L-1. After filtration, the unreacted AptAuRu nanoprobe in filtration solution exhibited strong catalytic effect on the slow particle rection of NaClO3-NaI-TDMAC, and the association complex particle appeared a resonance scattering peak at 472 nm. When the concentration of Pb2+ increased, the amount of AptAuRu nanoprobe in filtration solution decreased, and the catalysis weak, that result to the resonance scattering intensity at 472 nm decreasing. The decreased intensity ΔI472nm was linear to the Pb2+ concentration in the range of 0.12~60 pM, with a regression equation of ΔI472nm =3.1C + 7.3 and a detection limit of 0.08 pM Pb2+. The RS assay was applied to analysis of Pb2+ in waste water, with satisfactory results.

Key words: Pb2+, aptamer, AuRu nanoparticle, catalytic resonance scattering spectral assay

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