高等学校化学学报

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (11): 2162.

• 研究论文 • 上一篇    下一篇

纳米金与牛血清白蛋白作用的毛细管电泳研究

谢明一1, 郭振朋1, 陈义1,2   

  1. 1. 中国科学院化学研究所活体分析化学院重点实验室, 北京 100190;
    2. 北京分子科学国家实验室, 北京 100190
  • 收稿日期:2010-04-26 出版日期:2010-11-10 发布日期:2010-11-10
  • 通讯作者: 陈义, 男, 博士, 研究员, 博士生导师, 主要从事高效高通量生物微分析研究. E-mail: chenyi@iccas.ac.cn
  • 基金资助:

    国家自然科学基金(批准号: 90717120, 20905068)、国家“九七三”计划项目(批准号: 2007CB714504)和中国科学院知识创新工程重要方向项目(批准号: KJCX2-YW-H11)资助.

Inspection into the Interaction of Bovine Serum Albumin with Gold Nanoparticles by Capillary Electrophoresis

XIE Ming-Yi1, GUO Zhen-Peng1, CHEN Yi1,2*   

  1. 1. Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    2. Beijing National Laboratory of Molecular Science, Beijing 100190, China
  • Received:2010-04-26 Online:2010-11-10 Published:2010-11-10
  • Contact: CHEN Yi. E-mail: chenyi@iccas.ac.cn
  • Supported by:

    国家自然科学基金(批准号: 90717120, 20905068)、国家“九七三”计划项目(批准号: 2007CB714504)和中国科学院知识创新工程重要方向项目(批准号: KJCX2-YW-H11)资助.

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被引次数

14

摘要： 开展了针对微量纳米金与牛血清白蛋白相互作用的毛细管电泳研究, 测得二者的结合常数为28.6 L/μmol, 每个纳米金颗粒吸附约24个牛血清白蛋白分子. 结果表明, 牛血清白蛋白可改善并稳定纳米金的峰形, 二者作用时温育介质的pH以及电泳所用的缓冲溶液浓度对毛细管电泳(CE)效率有重要影响. 此法可推广到其它纳米颗粒的吸附研究中.

关键词: 毛细管电泳, 纳米金, 牛血清白蛋白, 作用常数

Abstract: A capillary electrophoretic method was developed to study the interaction of gold nanoparticles(GNPs) with bovine serum albumin(BSA), capable of determining the binding constant and binding capacity. For demonstration, citric acid-protected GNPs were tried, the measured bonding constant was 28.6 L/μmol and the saturated binding capacity was 24 BSA molecules per GNP, corresponding to a surface concentration of 4.5×1012 BSA/cm2. The determination was shown to depend on the incubating pH value and the concentration of run buffer which should be optimized accordingly. It was found that the peaks of the citric acid-protected GNPs were commonly broad, overlaid with irreproducible bars, but could easily be improved by addition of a small amount of BSA. The established method is expected to be extendable to inspecting the interaction of GNPs with other biomolecules.

Key words: Capillary electrophoresis, Gold nanoparticle, Bovine serum albumin, Interaction constant

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