高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (1): 28-34.doi: 10.7503/cjcu20160514

• 分析化学 • 上一篇    下一篇

羟丙基-β-环糊精对1-羟基芘与牛血清白蛋白相互作用的影响

张静1, 陈霖锋1, 朱亚先2, 张勇1()   

  1. 1. 近海海洋环境科学国家重点实验室, 厦门大学环境与生态学院, 厦门 361102
    2. 厦门大学化学化工学院, 厦门 361005
  • 收稿日期:2016-07-18 出版日期:2017-01-10 发布日期:2016-12-09
  • 作者简介:联系人简介: 张 勇, 男, 博士, 教授, 博士生导师, 主要从事环境化学方面的研究. E-mail: yzhang@xmu.edu.cn
  • 基金资助:
    国家自然科学基金(批准号: 21177102, 21577110)和高等学校博士学科点专项科研基金(批准号: 20130121130005)资助.

Effects of Hydroxypropyl-β-cyclodextrin(HPCD) on the Interaction of 1-Hydroxypyrene with Bovine Serum Albumin

ZHANG Jing1, CHEN Linfeng1, ZHU Yaxian2, ZHANG Yong1,*()   

  1. 1. State Key Laboratory of Marine Environmental Sciences of China (Xiamen University),College of Environment and Ecology, Xiamen University, Xiamen 361102, China
    2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
  • Received:2016-07-18 Online:2017-01-10 Published:2016-12-09
  • Contact: ZHANG Yong E-mail:yzhang@xmu.edu.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos. 21177102, 21577110) and the Specialized Research Fund for the Doctoral Program of Higher Education, China(No. 20130121130005).

摘要:

在模拟生理条件下, 利用荧光光谱、 圆二色光谱和紫外-可见吸收光谱探究了羟丙基-β-环糊精(HPCD)对1-羟基芘(1-OHPyr)与牛血清白蛋白(BSA)相互作用的影响及其相关机制. 结果表明, 291 K下HPCD可使1-OHPyr与BSA的结合常数降低为1.45×105 L/mol; 导致1-OHPyr-BSA体系中BSA的α-螺旋含量恢复, 色氨酸(TRP)残基周围微环境的极性变化减弱; 致使1-OHPyr及BSA的荧光寿命均延长, 且二者的结合距离增大. 初步研究结果表明, HPCD与1-OHPyr的包络作用是HPCD影响BSA与1-OHPyr结合的主要原因.

关键词: 1-羟基芘, 牛血清白蛋白, 羟丙基-β-环糊精, 光谱法, 影响机制

Abstract:

Fluorescence spectra, circular dichroism spectra and UV visible absorption spectra were employed to investigate the feasibility of regulating the interaction of 1-hydroxypyrene(1-OHPyr) with bovine serum albumin(BSA) using hydroxypropyl-β-cyclodextrin(HPCD) under simulated physiological conditions, and the related mechanism was preliminary discussed. The results showed that HPCD can inhibit the interaction of 1-OHPyr with BSA, and the binding constant of 1-OHPyr-BSA was decreased to 1.45×105 L/mol at 291 K. HPCD can also restore the helix of BSA and inhibit the polarity changes of the local microenvironment of tryptophan(TRP) residues in BSA. HPCD can increase the fluorescence lifetimes of 1-OHPyr and BSA in 1-OHPyr-BSA system, meanwhile increase the binding distance of 1-OHPyr with BSA. The inclusion behavior of HPCD with 1-OHPyr was primarily the main reason why HPCD affected the binding of 1-OHPyr with BSA.

Key words: 1-Hydroxypyrene, Bovine serum albumin, Hydroxypropyl-β-cyclodextrin, Spectroscopic method, Influencing mechanism

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