牛血清白蛋白介导合成的金纳米簇用于活细胞荧光成像

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (11): 2167.

牛血清白蛋白介导合成的金纳米簇用于活细胞荧光成像

袁媛1,2,4, 何晓晓1,2,4, 石慧2,3,4, 王柯敏2,3,4, 伍旭1,2,4, 霍希琴2,3,4

1. 湖南大学生物学院,
2. 化学生物传感与计量学国家重点实验室,
3. 化学化工学院,
4. 生物纳米与分子工程湖南省重点实验室, 长沙 410082

收稿日期:2010-04-16 出版日期:2010-11-10 发布日期:2010-11-10
通讯作者: 王柯敏. E-mail: kmwang@hnu.cn; 何晓晓. E-mail: xiaoxiaohe@hnu.cn
基金资助:
国家自然科学基金(批准号: 90606003, 20775021)、教育部创新研究群体项目、教育部科学技术重点项目(批准号: 107084)、新世纪优秀人才支持计划(批准号: NCET-06-0697)和湖南省自然科学基金创新研究群体项目(批准号: 10JJ7002)资助.

Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters

YUAN Yuan1,2,4, HE Xiao-Xiao1,2,4*, SHI Hui2,3,4, WANG Ke-Min2,3,4*, WU Xu1,2,4, HUO Xi-Qin2,3,4

1. College of Biology,
2. State Key Laboratory of Chemo/Biosensing and Chemometrics,
3. College of Chemistry and Chemical Engineering,
4. Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Hunan University, Changsha 410082, China

Received:2010-04-16 Online:2010-11-10 Published:2010-11-10
Contact: WANG Ke-Min. E-mail: kmwang@hnu.cn; HE Xiao-Xiao. E-mail: xiaoxiaohe@hnu.cn
Supported by:
国家自然科学基金(批准号: 90606003, 20775021)、教育部创新研究群体项目、教育部科学技术重点项目(批准号: 107084)、新世纪优秀人才支持计划(批准号: NCET-06-0697)和湖南省自然科学基金创新研究群体项目(批准号: 10JJ7002)资助.

摘要/Abstract

摘要： 以牛血清白蛋白介导合成金纳米簇, 并利用荧光分光光度计、纳米粒度及zeta电位仪以及非变性聚丙烯酰胺蛋白质电泳对其进行了表征. 结果表明, 该金纳米簇不仅荧光信号较强, 而且在不同pH值溶液中荧光稳定性好. 在此基础上进一步考察了金纳米簇与宫颈癌细胞(HeLa)间的相互作用. 结果表明, 该金纳米簇可成功进入活细胞内, 在最佳的培育时间和金纳米簇浓度条件下可达到较好的活细胞荧光标记效果, 且在经过细胞固定化处理后仍保持其标记形态.

关键词: 金纳米簇, 活细胞, 荧光标记

Abstract: Gold nanocluster was synthesized by bovine serum albumin directing, and was characterized by fluorescence spectrophotometer, zeta sizer and non-denaturing polyacrylamide gel electrophoresis. The results show that the gold nanocluster’s fluorescence signal is strong and stable at different pH value. The interaction between gold nanoclusters and HeLa cells was further studied. It demonstrates that the gold naoclusters could be successfully uptaken by living cells. The good effects of the fluorescent labeling of living cells can be achieved by optimal incubation time and gold nanoclusters’ concentration. Moreover, the fixation treatment did not change the form of labeling. These results provide some guidance to the further application of fluorescent gold nanoclusters in the field of live cells labeling.

Key words: Gold nanocluster, Living cell, Fluorescent labeling

TrendMD:

袁媛, 何晓晓, 石慧, 王柯敏, 伍旭, 霍希琴. 牛血清白蛋白介导合成的金纳米簇用于活细胞荧光成像. 高等学校化学学报, 2010, 31(11): 2167.

YUAN Yuan, HE Xiao-Xiao*, SHI Hui, WANG Ke-Min*, WU Xu, HUO Xi-Qin. Live Cells Fluorescence Imaging by Bovine Serum Albumin-mediated Synthesized Gold Nanoclusters. Chem. J. Chinese Universities, 2010, 31(11): 2167.

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