高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (6): 1116.

• 研究论文 • 上一篇    下一篇

给药硝酸钕后大鼠完整肝组织及肝组织提取物的NMR代谢组学研究

廖沛球1,2, 魏来1,2, 刘威3, 吴亦洁1, 李晓晶1, 倪嘉缵1, 裴奉奎1   

  1. 1. 中国科学院长春应用化学研究所, 长春 130022;
    2. 中国科学院研究生院, 北京 100049;
    3. 吉林省中医药科学院, 长春 130021
  • 收稿日期:2008-06-23 出版日期:2009-06-10 发布日期:2009-06-10
  • 通讯作者: 裴奉奎, 男, 研究员, 博士生导师, 主要从事现代波谱学研究, E-mail: peifk@ciac.jl.cn; 李晓晶, 女, 博士, 研究员, 主要从事现代波谱学应用研究, E-mail: xjli@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 20575065和20637010)资助.

Metabonomic Studies on Intact Livers and Liver Extracts from Rats Treated with Neodymium Nitrate

LIAO Pei-Qiu1,2, WEI Lai1,2, LIU Wei3, WU Yi-Jie1, LI Xiao-Jing1*, NI Jia-Zuan1, PEI Feng-Kui1*   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2.Graduate School of the Chinese Academy of Sciences, Beijing 100049, China;
    3.Jilin Provincial Academy of Traditional Chinese Medicine, Changchun 130021, China
  • Received:2008-06-23 Online:2009-06-10 Published:2009-06-10
  • Contact: LI Xiao-Jing, E-mail: peifk@ciac.jl.cn; PEI Feng-Kui, E-mail: xjli@ciac.jl.cn
  • Supported by:

    国家自然科学基金(批准号: 20575065和20637010)资助.

摘要:

通过分析不同给药剂量硝酸钕[Nd(NO3)3][2,10,50 mg/kg(体重)]后, 雄性Wistar大鼠完整肝组织的MAS 1H NMR谱和肝组织提取物的1H NMR谱, 结合肝组织病理切片图, 研究了稀土化合物Nd(NO3)3在大鼠体内的急性生物效应. 利用模式识别方法对给药Nd(NO3)3组和对照组大鼠肝组织1H NMR谱图数据进行了分析. 结果表明, 腹腔注射Nd(NO3)3后, 大鼠肝脏中甘油三酯、亮氨酸(异亮氨酸)、乳酸、丙氨酸、丙酮酸、磷酸胆碱和葡萄糖含量升高, 氮氧三甲胺含量降低. 肝脏病理图显示, 50 mg/kg(体重)组大鼠肝细胞可见微小坏死灶和门管区炎细胞轻度增多. 推测硝酸钕能影响大鼠肝脏中能量代谢(糖代谢和脂肪代谢)和氨基酸代谢, 对大鼠肝脏造成损伤, 且其损伤程度随剂量的增加有增强趋势.

关键词: 核磁共振, 代谢组学, 模式识别, 肝, 硝酸钕, 生物效应

Abstract:

The biochemical effects of neodymium nitrate were studied by NMR spectroscopic-based metabonomic approach. Male Wistar rats were treated intraperitoneally with different doses(2, 10 and 50 mg/kg body weight) of Nd(NO3)3. Liver samples from Nd3+-treated rats were analyzed either by 600 MHz 1H MAS NMR techniques for intact liver or using high resolution(liquid state) 1H NMR for liver chloroform/water/methanol extracts. All NMR spectra data were analyzed by pattern recognition using principal components analysis. Liver lesions induced by Nd(NO3)3 were represented by increased triglyceride, leucine(isoleucine), lactate, alanine, pyruvate, phosphocholine and glucose concentration, and reduced trimethylamine N-oxide(TMAO) concentration in liver. Histopathology examination showed cell necrosis and inflammation in the liver of 50 mg/kg body weight dosed rats. It was demonstrated that neodymium nitrate could cause damages to rats liver by disturbing the energy metabolism(glucose and lipid metabolism) and amino acid metabolism in the liver.

Key words: Nuclear magnetic resonance, Metabonomics, Pattern recognition, Liver, Neodymium nitrate, Biochemical effect

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