痕量甲胎蛋白的免疫纳米金催化-氧化亚铜微粒共振散射光谱分析

高等学校化学学报 ›› 2009, Vol. 30 ›› Issue (6): 1109.

痕量甲胎蛋白的免疫纳米金催化-氧化亚铜微粒共振散射光谱分析

蒋治良¹, 张玉兰¹, 梁爱惠², 韦丽丽¹, 王素梅²

1. 广西师范大学环境与资源学院, 广西环境工程与保护评价重点实验室, 桂林 541004;
2. 桂林工学院材料与化学工程系, 桂林 541001

收稿日期:2008-10-10 出版日期:2009-06-10 发布日期:2009-06-10
通讯作者: 蒋治良, 男, 博士, 教授, 主要从事纳米材料化学与光谱分析研究. E-mail: zljiang@mailbox.gxnu.edu.cn
基金资助:
国家自然科学基金(批准号: 20667001, 20865002)、广西新世纪十百千人才资助计划基金和广西环境工程与保护评价重点实验室项目(批准号: 桂科能0702Z019)资助.

Immunonanogold Catalytic-Cu₂O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein

JIANG Zhi-Liang¹*, ZHANG Yu-Lan¹, LIANG Ai-Hui², WEI Li-Li1, WANG Su-Mei²

1. Guangxi Key Laboratory of Enviromental Engineering, Protection and Assessment, School of Enviroment, Guangxi Normal University, Guilin 541004, China;
2. Department of Material and Chemical Engineering, Guilin University of Technology, Guilin 541004, China

Received:2008-10-10 Online:2009-06-10 Published:2009-06-10
Contact: JIANG Zhi-Liang, E-mail: zljiang@mailbox.gxnu.edu.cn
Supported by:
国家自然科学基金(批准号: 20667001, 20865002)、广西新世纪十百千人才资助计划基金和广西环境工程与保护评价重点实验室项目(批准号: 桂科能0702Z019)资助.

摘要/Abstract

摘要：

用粒径15 nm 的纳米金标记单克隆羊抗人甲胎蛋白(GAFP), 制备了甲胎蛋白(AFP)的免疫纳米金探针(AuGAFP). 纳米金及AuGAFP均对葡萄糖还原铜(Ⅱ)生成Cu₂O微粒这一慢反应具有较强的催化作用, Cu₂O微粒在620 nm处产生1个较强的共振散射峰. 将AFP-AuGAFP免疫反应与离心分离技术结合, 建立了超痕量AFP的免疫纳米金催化-Cu₂O微粒共振散射光谱新方法. 随着AFP浓度的增大, AFP-AuGAFP免疫复合物微粒增多, 离心液中AuGAFP浓度降低, 620 nm处的共振散射光强度I_{620 nm}线性降低, 其降低值ΔI_RS与AFP质量浓度ρ(AFP)在0.10～16.0 ng/mL范围内呈现良好的线性关系, 其回归方程为ΔI_RS=4.27ρ(AFP)+1.28, 检出限为0.05 ng/mL. 本方法所用试剂易得, 反应易控制, 灵敏度高, 选择性好, 用于定量分析人血清中的AFP, 结果令人满意.

关键词: 甲胎蛋白, 纳米催化, 免疫纳米金, 氧化亚铜微粒, 共振散射光谱法

Abstract:

15 nm-nanogold was used to label monoclone goat antihuman α-fetoprotein antibody(GAFP) to obtain immunonanogold probe(AuGAFP) for α-fetoprotein(AFP). Both nanogold and the probe have catalytic effect on the slow Cu₂O particle reaction between Fehling reagent and glucose that exhibit a resonance scattering peak at 620 nm. Combining AFP-AuGAFP immunoreaction with centrifugation technique, a highly sensitive immunonanogold catalytic-Cu₂O particle resonance scattering spectral assay for AFP was proposed. With addition of AFP, the AFP-AuGAFP immunocomplex increased, the excess probe in the supernatant decreased, and the resonance scattering intensity at 620 nm decreased linearly. The decreased intensity ΔI_RS was linear to AFP concentration[ρ(AFP)] in the range of 0.10—16.0 ng/mL, with a regression equation of ΔI_RS=4.27ρ(AFP)+1.28, and a detection limit of 0.05 ng/mL. This method was applied to the detection of AFP in sera, with high sensitivity and good selectivity, in addition to low-cost reagents and easy controlling reaction.

Key words: α-Fetoprotein, Nanocatalysis, Immunonanogold, Cu₂O particle, Resonance scattering spectral assay

TrendMD:

蒋治良, 张玉兰, 梁爱惠, 韦丽丽, 王素梅. 痕量甲胎蛋白的免疫纳米金催化-氧化亚铜微粒共振散射光谱分析. 高等学校化学学报, 2009, 30(6): 1109.

JIANG Zhi-Liang*, ZHANG Yu-Lan, LIANG Ai-Hui, WEI Li-Li, WANG Su-Mei. Immunonanogold Catalytic-Cu₂O Particle Resonance Scattering Spectral Determination of Trace α-Fetoprotein. Chem. J. Chinese Universities, 2009, 30(6): 1109.

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