高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

金纳米粒子与单链DNA的相互作用

孙莉萍1, 张建锋1, 李辉1, 王秀燕1, 张召武1, 王霜1, 张其清1,2   

    1. 厦门大学材料学院生物医学工程研究中心, 厦门市生物医学工程技术研究中心, 福建省生物医学工程重点实验室, 厦门 361005;
    2. 中国医学科学院中国协和医科大学生物医学工程研究所, 天津市生物医学材料重点实验室, 天津 300192
  • 收稿日期:2008-04-22 修回日期:1900-01-01 出版日期:2009-01-10 发布日期:2009-01-10
  • 通讯作者: 孙莉萍, 张其清

Interaction Between Gold Nanoparticles and ssDNA

SUN Li-Ping1*, ZHANG Jian-Feng1, LI Hui1, WANG Xiu-Yan1, ZHANG Zhao-Wu1, WANG-Shuang1, ZHANG Qi-Qing1,2*   

    1. Research Center of Biomedical Engineering, College of Materials, Xiamen University, Technology Research Center of Biomedical Engineering of Xiamen, Key Laboratory of Biomedical Engineering of Fujian, Xiamen 361005, China;
    2. Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China
  • Received:2008-04-22 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10
  • Contact: SUN Li-Ping, ZHANG Qi-Qing

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被引次数

24

摘要： 研究了金纳米粒子与单链DNA在不同pH值时的相互作用以及金纳米粒子与不同碱基序列单链DNA的相互作用. 结果表明, 在pH为12.6的强碱性条件下, 单链DNA能使金纳米粒子稳定分散在溶液中; 在pH为1.4的强酸性条件下, 单链DNA能保护金纳米粒子不发生融合, 而只发生团聚, 且团聚现象具有可逆性. 不同寡核苷酸对金纳米粒子的亲和力按poly dA>poly dC>poly dT的顺序依次减弱. 单链DNA对纳米金的保护作用强度与单链DNA的长度成正比.

关键词: 金纳米粒子, 单链DNA, 相互作用, ssDNA

Abstract: The interaction between unmodified gold nanoparticles and single-stranded DNA (ssDNA) was studied at different pH value. And the sequence-dependent stability of ssDNA-GNPs complex was investigated. GNPs precipitated from red colloid solution in the form of aggregation after alkalization with NaOH or dissolve after acidification with HCl. Both processes were irreversible. If the same particles were incubated with ssDNA, ssDNA-GNPs complex were stable against NaOH-induced aggregation at pH=12.6, and aggregated at pH=1.4, while redispersed at pH=12.6. It was concluded that unmodified GNPs could be coated by unmodified ssDNA, which protected GNPs dispersing in solution at pH=12.6, and prevented GNPs from dissolving at pH=1.4, as measured by TEM and UV-Vis absorption spectrum. The bindig affinities of oligonucleotides to GNPs were different in the order of poly dA>poly dC>poly dT. Moreover, longer ssDNA had stronger protective effect to gold nanoparticles. HCl-induced GNPs aggregation could be an effective method to identify the diversity of deoxyribonucleotides in ssDNA sequences.

Key words: Gold nanoparticle, Single-stranded DNA, Interaction, ssDNA

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