高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

手性金属配合物d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层模型的转运吸收

牟永平1, 吴刚1,4, 乔秀文2, 高愈希3, 周立社1, 和彦苓4   

    1. 包头医学院生物化学与分子生物学教研室, 包头 014010;
    2. 石河子大学化学化工学院, 石河子 832002;
    3. 中国科学院高能物理研究所核分析技术重点实验室, 北京 100049;
    4. 包头医学院生物微量元素研究所, 包头 014010
  • 收稿日期:2007-11-02 修回日期:1900-01-01 出版日期:2008-07-10 发布日期:2008-07-10
  • 通讯作者: 吴刚

Transport and Absorption of Chiral Metal Complexes d-[Co(EDTA)]- and l-[Co(EDTA)]- Across Caco-2 Cell Monolayers

MU Yong-Ping1, WU Gang1,4*, QIAO Xiu-Wen2, GAO Yu-Xi3, ZHOU Li-She1 , HE Yan-Ling4   

    1. Department of Biochemistry and Molecular Biology, Baotou Medical College, Baotou 014010, China;
    2. School of Chemistry and Chemical Engineering, Shihezi University, Shihezi 832002, China;
    3. Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;
    4. The Research Institute of Trace Element in Organism, Baotou Medical College, Baotou 014010, China
  • Received:2007-11-02 Revised:1900-01-01 Online:2008-07-10 Published:2008-07-10
  • Contact: WU Gang

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摘要： 采用动力学研究方法测定了手性金属配合物d-[Co(EDTA)]-和l-[Co(EDTA)]-跨Caco-2细胞单层的转运速率. 研究发现, 由于金属配合物的手性差异, 导致d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层的转运速率明显不同; 手性金属配合物经Caco-2细胞单层转运吸收时存在手性选择性, 表明小肠对手性金属配合物药物可能有选择性吸收; d-[Co(EDTA)]-和l-[Co(EDTA)]-经Caco-2细胞单层的转运依赖浓度梯度驱动, 说明该对映体配合物经Caco-2细胞单层转运吸收时存在简单扩散的转运方式.

关键词: 手性, 金属配合物, Caco-2细胞单层, 转运, 吸收

Abstract: The trasport rate of chiral complexes d-[Co(EDTA)]- and l-[Co(EDTA)]- across Caco-2 cell monolayers were measured by the kinetics method. The studies discover that there is an obvious difference about the trasport rate of d-[Co(EDTA)]- and l-[Co(EDTA)]- when they get across Caco-2 cell monolayers because of the chiral difference of the metal complexes. The results show that there is a chiral selectivity when chiral metal complexes were transported and absorbed across Caco-2 cell monolayers and imply that intestine would selectively absorb drugs of chiral metal complexes. The trasport rate of d-[Co(EDTA)]- and l-[Co(EDTA)]- passing through Caco-2 cell monolayers depends on the grads of the complexes concentration, it is shown that their transport model is as simple diffuse when they were transported and absorbed through Caco-2 cell monolayers.

Key words: Chirality, Metal complex, Caco-2 cell monolayer, Transpot, Absorption

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