高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

铁氰化钾-鲁米诺体系后化学发光反应及其分析应用研究
-----分子印迹-后化学发光法测定双嘧达莫

刘清慧; 吕九如; 冯娜   

  1. 陕西师范大学化学与材料科学学院, 西安 710062
  • 收稿日期:2005-09-01 修回日期:1900-01-01 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 吕九如

Post Chemiluminescence Reaction of Dipyridamole in Luminol in Luminol-potassium Ferricyanide System and Its Application in Analysis
-----The Molecular Imprinting-post Chemiluminescene Method for the Determination of Dipyridamole

LIU Qing-Hui; LU Jiu-Ru*; FENG Na   

  1. College of Chemistry and Material Science, Shanxi Normal University, Xi′an 710062, China
  • Received:2005-09-01 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: LU Jiu-Ru

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摘要： 研究了双嘧达莫在铁氰化钾-鲁米诺化学发光反应体系中的后化学发光反应, 并在研究其反应的动力学性质、化学发光光谱、荧光光谱以及一些相关问题的基础上, 探讨了反应机理; 合成了双嘧达莫的分子印迹聚合物, 以此聚合物为分子识别物质, 利用铁氰化钾-鲁米诺-双嘧达莫后化学发光体系, 建立了测定双嘧达莫的高选择性分子印迹-后化学发光分析方法. 所建方法的线性范围为1.0×10-8-1.0×10-6 g/mL(r=0.999 2), 检出限为3×10-9 g/mL, 相对标准偏差为2.7%(1.0×10-7 g/mL双嘧达莫, n=11).

关键词: 后化学发光, 分子印迹, 流动注射, 双嘧达莫

Abstract: In this work, a new post chemiluminescence(PCL) reaction of the Dipyridamole in the potassium ferricyanide-luminol system was found. A possible PCL mechanism was proposed based upon the studies of the kinetics characteristics, the CL spectra, fluorescence spectra of some relative reactions and other series of experiments. At the same time, Dipyridamole-imprinted polymer(MIP) was synthesized. Using the MIP as the recognition material and employing potassium ferricyanide-luminol-Dipyridamole PCL system, a new molecular imprinting-post-chemiluminescence(MIP-PCL) method for the determination of Dipyridamole was established. The linear range of this method was 1.0×10-8-1.0×10-6 g/mL with a linear correlation coefficient of 0.999 2, the detection limit was 3×10-9 g/mL and the relative standard deviation for the determination of 1.0×10-7 g/mL Dipyridamole solution was 2.7%(n=11). This method was successfully applied to the determination of Dipyridamole in human urine with a satisfactory result.

Key words: Post-chemiluminescence, Molecular imprinting, Flow injection, Dipyridamole

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