高等学校化学学报

高等学校化学学报

• 研究论文 • 上一篇    下一篇

自组装表面等离子体子共振传感器用于测定抗生素-蛋白结合率的研究

邹向宇, 周建光, 谢萍, 张磊, 汪淑华, 冯国栋, 金钦汉   

  1. 吉林大学化学学院微分析仪器研究所, 吉林省光谱分析仪器工程技术研究中心, 长春 130023
  • 收稿日期:2005-10-08 修回日期:1900-01-01 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 金钦汉

Studies on Determination of Percentage Bound of Eight Antibiotics by Using a New Home-made Surface Plasomon Resonace Sensor

ZOU Xiang-Yu, ZHOU Jian-Guang, XIE Ping, ZHANG Lei, WANG Shu-Hua,
FENG Guo-Dong, JIN Qin-Han*   

  1. Institute for Miniature Instrumentation, College of Chemistry, Jilin Province
    Research Center for Enginerring and Technology of Spectral Instruments, Jilin University, Changchun 130021, China
  • Received:2005-10-08 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: JIN Qing-Han

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被引次数

6

摘要： 自行设计组装了一套以白色发光二极管为光源的新型表面等离子体子共振传感器, 并采用此传感器通过测量动力学参数测定了8种抗生素与固定在金膜上的牛血清白蛋白之间的结合率, 将所得数据与常用的平衡透析法进行了比较, 两者具有良好的可比性和线性相关性, 说明采用此法也可测定抗生素与蛋白的结合率. 该方法具有测定周期短、 样品用量少、 操作简便、 预测效果良好以及仪器易于小型化甚至微型化等优点.

关键词: 表面等离子体共振传感器, 抗生素, 蛋白, 结合率

Abstract: Based on surface plasmon resonance (SPR), a novel optical chemical sensor was developed. Traditionally, wavelength modulation SPR sensing method adapted tungsten lamp as the light source with an expensive and complicated apparatus. The device presented here is a relatively simple and inexpensive one. It was designed using a white LED with a 5 V battery as the light source. The percentage bound of 8 antibiotics with BSA was studied by this setup. The results were compared with the reliable percentage bound data obtained by standard equilibrium dialysis and a relative good agreement was achieved.

Key words: Surface plasomon resonance sensor, Antibiotic, Protein, Percentage bound

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