高等学校化学学报

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光散射法快速灵敏测定和表征啤酒酵母

陈绍芬; 黄承志; 谭克俊   

  1. 西南大学化学化工学院, 重庆 400715
  • 收稿日期:2005-11-20 修回日期:1900-01-01 出版日期:2006-06-10 发布日期:2006-06-10
  • 通讯作者: 黄承志

Rapid and Sensitive Detection and Characterization of Saccharomyces
cerevisiae by Light Scattering Method

CHEN Shao-Fen; HUANG Cheng-Zhi*; TAN Ke-Jun   

  1. College of Chemistry and Chemical Engineering, Southwest Normal University,
    Chongqing 400715, China
  • Received:2005-11-20 Revised:1900-01-01 Online:2006-06-10 Published:2006-06-10
  • Contact: HUANG Cheng-Zhi

摘要: 研究了啤酒酵母的光散射光谱. 结果表明, 在波长308.0 nm处, 光散射强度与啤酒酵母浓度在2.0×104-2.0×106 Cell/mL 范围内呈线性关系, 检出限(3σ)为4.94×102 Cell/mL. 将此方法成功地应用于培养液中啤酒酵母含量的快速、 灵敏的测定, 并对其散射光谱进行了表征.

关键词: 光散射分析法, 散射截面, 吸收截面, 啤酒酵母, 生物量

Abstract: The light scattering(LS) spectra of Saccharomyces cerevisiae was studied. The results show that the LS intensities were in proportion to the concentrations of Saccharomyces cerevisiae at peak 308.0 nm in the concentration range of 2.0×104-2.0×106 Cell/mL. The detection limit(3σ) was 4.94x102 Cell/mL. The present new method was successfully applied to the rapid, sensitive determination and characterization of Saccharomyces cerevisiae biomass in suspensions.

Key words: Light scattering assay, Scattering cross section, Absorption cross section, Saccharomyces cerevisiae, Biomass

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