高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (7): 1252.

• 研究简报 • 上一篇    下一篇

无胶筛分芯片电泳研究贝类毒素-软骨藻酸与寡聚DNA的相互作用

周小棉1,2, 李大志1,3, 沈铮1, 刘伟3, 李桂茹4, 林炳承1   

  1. 1. 中国科学院大连化学物理研究所,大连116023;
    2. 广州医学院附属广州市第一人民医院检验科,广州510500;
    3. 大连海关分析测试中心,大连116001;
    4. 大连医科大学附属第二医院,大连116023
  • 收稿日期:2004-09-04 出版日期:2005-07-10 发布日期:2005-07-10
  • 通讯作者: 林炳承(1944年出生),男,研究员,博士生导师,从事毛细管电泳和芯片实验室研究.E-mail:bclin@dicp.ac.cn E-mail:bclin@dicp.ac.cn
  • 基金资助:

    国家自然科学基金(批准号:20275039);国家自然科学基金重点项目(批准号:20035010);国家自然科学基金重大项目(批准号:20299030);中国科学院仪器研制专项基金资助

Interaction Between Domoic Acid and Double-Stranded Oligodeoxy-nucleotide Studied by Using Microchip-based Non-gel Sieving Electrophoresis

ZHOU Xiao-Mian1,2, LI Da-Zhi1,3, SHEN Zheng1, LIU Wei3, LI Gui-Ru4, LIN Bing-Cheng1   

  1. 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
    2. Department of Clinical Laboratory, Guangzhou First Municipal People's Hospital, Affiliated of Guangzhou Medical College, Guangzhou 510500, China;
    3. Dalian Customs Laboratory, Dalian 116001, China;
    4. The Second Affiliated Hospital, Dalian Medical University, Dalian 116023, China
  • Received:2004-09-04 Online:2005-07-10 Published:2005-07-10

PDF (PC)

被引次数

12

关键词: 无胶筛分芯片电泳, 相互作用, 软骨藻酸, 双链DNA

Abstract: The interaction between domoic acid(DA) and 18-mer double-stranded DNA(dsDNA) was investigated by using microchip-based non-gel sieving electrophoresis. For the 18-mer dsDNA with a sequence of 3'-GCATTGGTTGACGTTGCA-5', the amount of free dsDNA decreases with the increase of the added DA and also with the increase of reactive time. Meanwhile, the newly-produced two peaks were observed. The experimental results show that there is a strong interaction between DA and the 18-mer dsDNA. DA makes the degradation of the 18-mer dsDNA. The non-gel sieving electrophoresis on microchip is a rapid, convenient, highly sensitive method for studying the interaction between DNA and small molecules.

Key words: Microchip non-sieving electrophoresis, Interaction, Domoic acid, 18-mer dsDNA

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