高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 852.

• 研究论文 • 上一篇    下一篇

菱角中抗肿瘤活性成分的分离、提纯及结构鉴定

牛凤兰1, 尹建元2, 董威严1, 王学东1   

  1. 1. 吉林大学公共卫生学院;
    2. 吉林大学药学院, 长春130021
  • 收稿日期:2004-09-16 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 牛凤兰(1951年出生),女,教授,主要从事药食同用植物与健康的研究.E-mail:zkeb@sina.com E-mail:zkeb@sina.com
  • 基金资助:

    吉林省科学技术厅科研项目(批准号:20030226-3)资助.

Separation, Purification and Structural Identification of Anti-tumour Compound in Trapa manshurica Fler

NIU Feng-Lan1, YIN Jian-Yuan2, DONG Wei-Yan1, WANG Xue-Dong1   

  1. 1. School of Public Health;
    2. College of Pharmacy, Jilin University, Changchun 130021, China
  • Received:2004-09-16 Online:2005-05-10 Published:2005-05-10

PDF (PC)

被引次数

102

摘要： 采用柱层析分离技术,以硅胶为填料,以石油醚-醋酸乙酯-甲酸(体积比为5∶4∶0.1)为展开剂(Rf=0.4),提取并分离菱角中具有抗肿瘤作用的活性成分,经质谱法(MS)、红外光谱法(IR)及核磁共振法(1HNMR、13CNMR)对分离得到的纯品进行了结构鉴定,确定该化合物为3,4,5-三羟基苯甲酸的二聚体,同时采用四甲基偶氮唑盐(MTT)还原法、光学显微镜及透射电子显微镜等方法对其抗癌机理进行初步探讨,实验结果表明,该化合物具有诱导肿瘤细胞凋亡的抗肿瘤作用.

关键词: 菱角, 3, 4, 5-三羟基苯甲酸二聚体, 抗肿瘤, 结构鉴定

Abstract: The anti-tumour compound in Trapa manshurica Fler was separated and purified by using the silica gel column chromatography, with an eluting agent of petroleum ether-ethyl acetate-formic acid(volume ratio is 5∶4∶0.1, Rf=0.4). The structure of compound Ⅰ obtained was identified to be 3,4,5-trihydroxybenzoic acid dimmer by means of MS, IR, 1H NMR and 13C NMR, and the preliminary study shows that it has a quite high activity for inhibiting the proliferation of hela cell induction apoptosis to anti-tumor mechanism by means of MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] reduction method, optical microscope, transmission electron microscope and flow cytometry.

Key words: Trapa manshurica Fler, 3-(4,5-Dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide, Anti-tumour, Structural identification

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