高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (5): 846.

• 研究论文 • 上一篇    下一篇

hKv4.3最小功能启动子区与上游抑制元件分析

李皓1,2, 姜春来1, 于湘晖1, 吴永革1, 李惟1, 孔维1   

  1. 1. 吉林大学生命科学学院, 长春130021;
    2. 吉林工程技术师范学院, 长春130052
  • 收稿日期:2004-11-04 出版日期:2005-05-10 发布日期:2005-05-10
  • 通讯作者: 孔　维(1965年出生),男,博士,教授,博士生导师,从事基因疫苗研究.E-mail:weikong@mail.jlu.edu.cn E-mail:weikong@mail.jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:30170215)资助.

Analysis of the Minimal Functional Promoter Region for hKv4.3 and Upstream Suppressors

LI Hao1,2, JIANG Chun-Lai1, YU Xiang-Hui1, WU Yong-Ge1, LI Wei1, KONG Wei1   

  1. 1. College of Life Science, Jilin University, Changchun 130012, China;
    2. Jilin Teacher's Institute of Engineering Technology, Changchun 130052, China
  • Received:2004-11-04 Online:2005-05-10 Published:2005-05-10

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被引次数

3

摘要： 通过克隆hKv4.3的启动子区和相关的上游调控元件,对hKv4.3基因在转录水平的调控进行了分析.对启动子区5'端一系列的删除突变分析证明,hKv4.3基因的最小功能启动子区是位于转录起始位点附近的-156～+2bp序列.经序列分析发现,这个启动子缺乏典型的TATA-box,却存在另外3个元件,即E-box(CANNTG),CArG-box[CC(A/T)6GG]和CACC-box(GGTGC),其中CArG-box对该启动子活性起关键作用.同时在启动子区找到一个未见报道的大小为10bp的抑制子T,删除抑制子T,则启动子活性增加1倍以上.

关键词: hKv4.3, 最小功能启动子区, 抑制子

Abstract: Activation(depolarization) and recovery(repolarization) of the heart is the result of the orchestrated activity of a variety of ion channels and transporters. Potassium currents such as the transient outward potassium current, Ito, contribute significantly to phase 1 of repolarization. hKv4.3 is the predominant gene that is believed to underlie Ito in the human ventricle. The regulation of hKv4.3 at the transcriptional level was examined by cloning its promoter region and associated negative regulatory elements. Serial deletions of the 5' flanking region identified the minimal promoter of hKv4.3 at-156 to +2 bp surrounding the transcriptional start site. The promoter is not cell type specific and lacks a canonical TATA box. Three motifs found within the promoter include an E-box(CANNTG), a CArG-box[CC(A/T)6GG], and a CACC-box. The CArG-box is necessary for transcriptional activity. A novel repressor element named T was identified. The deletion of the T element will at least double promoter activity.

Key words: hKv4.3, Minimal promoter, Repressor

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