高等学校化学学报

高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (4): 660.

• 研究简报 • 上一篇    下一篇

基质中加入少量盐类改善MALDI-TOF MS的谱图信噪比

方彩云1, 翟建军1, 刘锋1,2, 杨芃原1   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 国家人类基因组南方基因中心, 上海 201203
  • 收稿日期:2004-05-25 出版日期:2005-04-10 发布日期:2005-04-10
  • 通讯作者: 杨原(1949年出生),男,博士,教授,博士生导师,从事蛋白质组学研究.E-mail:pyyang@fudan.edu.cn E-mail:pyyang@fudan.edu.cn
  • 基金资助:

    国家重点基础研究计划项目(批准号:01CB510202)资助.

Improvement of Signal to Noise Ratio in MALDI-TOF MS with Addition of Salts to the Matrix

FANG Cai-Yun1, ZHAI Jian-Jun1, LIU Feng1,2, YANG Peng-Yuan1   

  1. 1. Chemistry Department, Fudan University, Shanghai 200433, China;
    2. Chinese National Human Genome Center at Shanghai, Shanghai 201203, China
  • Received:2004-05-25 Online:2005-04-10 Published:2005-04-10

PDF (PC)

被引次数

15

关键词: 盐, 基质加合物, 信噪比, MALDI-TOFMS

Abstract: CHCA as a matrix facilitates the ionization of proteins and peptides in a matrix-assisted laser desorption/ionization(MALDI) time-of-flight(TOF) mass spectrometer. The matrix itself also ionizes and so do its adducts. Matrix clusters and adducts interfere with peptide ionization and peptide mass spectrum interpretation, especially at low sample concentrations and within m/z 500-1000 range. These matrix adducts, especially within m/z 500-1000, are significantly reduced with addition of ammonium citrate, ammonium sulfate, ammonium benzoate, ammonium bicarbonate, ammonium citrate and potassium dihydrogen phosphate to the matrix and the samples were deposited onto the MALDI target. The reduction of matrix adducts improves peptide ionization and signal-to-noise ratio, which enhances the reliability of database searching.

Key words: Salt, Matrix adduct, S/N ratio, MALDI-TOF MS

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