高等学校化学学报 ›› 2005, Vol. 26 ›› Issue (10): 1825-1828.

• 研究论文 • 上一篇    下一篇

肌红蛋白-纳米氧化铝模板-金胶复合组装体的直接电化学与电催化

关晓辉1, 秦玉华1,2, 张术勇1, 郭军1, 李景虹2   

  1. 1. 东北电力学院应用化学系,吉林132012;
    2. 中国科学院长春应用化学研究所,长春130024
  • 收稿日期:2004-09-29 出版日期:2005-10-10 发布日期:2005-10-10
  • 基金资助:

    国家自然科学基金(批准号:20125513)资助.

Direct Electrochemistry and Electrocatalysis of Myoglobin Immobilized on Nano-alumina-gold Colloid Assembly System

GUAN Xiao-Hui1, QIN Yu-Hua1,2, ZHANG Shu-Yong1, GUO Jun1, LI Jing-Hong2   

  1. 1. Department of Applied Chemistry,Northeast China Institute of Electric Power Engineering,Jilin 132012,China;
    2. State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China
  • Received:2004-09-29 Online:2005-10-10 Published:2005-10-10

摘要: 将肌红蛋白(Mb)固定在纳米氧化铝(AAO)模板-金胶复合组装体修饰玻碳电极表面,制得Mb/AAO/Au colloid/GC薄膜电极.在pH=5.4的HAc-NaAc缓冲溶液中,该薄膜电极于-0.21 V(vs.Ag/AgC l)处有一对准可逆的氧化还原峰,为Mb血红素辅基Fe(Ⅲ)/Fe(Ⅱ)电对的特征峰.在AAO/Au colloid薄膜的微环境中,Mb与玻碳电极间的电子传递明显加快,该Mb/AAO/Au colloid/GC薄膜电极还可用于过氧化氢和溶解氧的催化还原.

关键词: 肌红蛋白, 纳米氧化铝, 金胶, 直接电化学, 电催化

Abstract: Mb/AAO/Au colloid /GC electrodes were made by immobilizing Mb on the surface of Au colloid modified glassy carbon electrode covered with AAO templates.The cyclic voltammgram of Mb/AAO/Au colloid/GC films shows a pair of well-defined and nearly reversible peaks at about-0.21 V(vs.Ag/AgCl) in acetic acid-sodium acetate buffers with pH=5.4,being the characteristic peaks of the Mb heme Fe(Ⅲ)/Fe(Ⅱ) redox couples.In the microenvironment of the AAO/Au colloid film,the electron transfer between Mb and the electrode was greatly enhanced.The hydrogen peroxide and oxygen were catalytically reduced at(Mb/AAO/Au) colloid/GC electrodes.

Key words: Myoglobin(Mb), Nano-alumina, Gold colloid, Direct electrochemistry, Electrocatalysis

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