高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (7): 1277.

• 研究论文 • 上一篇    下一篇

IgG配基对肿瘤坏死因子α的吸附机制研究

魏佼, 方晖, 孔德领, 俞耀庭   

  1. 南开大学生物活性材料教育部重点实验室, 天津 300071
  • 收稿日期:2003-05-22 出版日期:2004-07-24 发布日期:2004-07-24
  • 通讯作者: 俞耀庭(1932年出生),男,教授,博士生导师,主要从事高分子化学与物理和生物医学材料研究.E-mail:yaoting@public.tpt.tj.cn E-mail:yaoting@public.tpt.tj.cn
  • 基金资助:

    国家自然科学基金(批准号:30000042);天津大学基金;南开大学基金;中英合作基金资助

Studies on Adsorption Mechanism of TNFα with IgG Ligand

WEI Jiao, FANG Hui, KONG De-Ling, YU Yao-Ting   

  1. Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China
  • Received:2003-05-22 Online:2004-07-24 Published:2004-07-24

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摘要： 以球形琼脂凝胶为载体,键连免疫球蛋白IgG配基,通过化学修饰等方法,研究了IgG与TNFα的亲和作用机制,进而推测出吸附作用位点.结果显示,IgG分子的氨基、羧基及色氨酸残基中的吲哚基可能影响吸附作用;IgG配基连接手臂可以提高吸附性能,在3000U/mLTNFα血浆中,采用己二胺手臂吸附量可达7084.68U/mL.鉴于IgG配基的高效特异性,研究结果不仅具有一定的理论意义,也有较强的临床应用前景

关键词: 肿瘤坏死因子α, IgG, 修饰, 机制

Abstract: Tumor necrosis factor α(TNFα) is recognized as the principal inflammatory mediator in the pathogenesis of endotoxemia and dyscracia. Removal of TNFα with immunoadsorbent is one of the focus in the study of anti-TNFα therapy. In the present study, γ-globulin IgG was immobilized onto agarose beads to produce a new type TNFα adsorbent. The affinity mechanism of IgG ligand was studied by means of IgG molecule modification with chemical reagents. The results showed that amino group had a significant influence on the adsorption capacity; carboxyl and indole groups had a negative effect; guanidine and imidazole groups had no effect on adsorption capacity. Furthermore, 1,6-hexamethylene diamine(DAH) was used as a spacer to link agar gel support and IgG ligand which was favourable for reducing the steric hindrance and elevating the adsorption capacity. The IgG immobilized adsorbent showed a strong specificity as well as high efficacy and therefore had a potential for clinical application.

Key words: TNFα, IgG, Modification, Mechanism

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