高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (6): 1056.

• 研究论文 • 上一篇    下一篇

亲和吸附剂对细菌内毒素吸附性能的研究

方晖, 魏佼, 俞耀庭   

  1. 南开大学生物活性材料教育部重点实验室, 天津 300071
  • 收稿日期:2003-05-13 出版日期:2004-06-24 发布日期:2004-06-24
  • 通讯作者: 俞耀庭(1932年出生),男,教授,博士生导师,主要从事高分子化学与物理和生物医学材料研究.E-mail:yaoting@public.tpt.t.jcn E-mail:yaoting@public.tpt.t.jcn
  • 基金资助:

    国家自然科学基金(批准号:30000042);天津市自然科学基金(批准号:023613411);天津大学和南开大学共建基金;中英合作基金资助

Studies on Adsorption Performance of Affinity Adsorbent for Bacterial Endotoxins

FANG Hui, WEI Jiao, YU Yao-Ting   

  1. Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071, China
  • Received:2003-05-13 Online:2004-06-24 Published:2004-06-24

PDF (PC)

被引次数

27

摘要： 制备了以球形纤维素为载体、8种氨基酸和1种聚赖氨酸为配基的吸附剂,对质量浓度为100.0pg/mL的内毒素水溶液进行了吸附研究,绘制了吸附等温线,并初步探讨了吸附机理.结果表明,精氨酸和赖氨酸配基具有良好的吸附能力,在1.5mL100.0pg/mL内毒素溶液中吸附量分别达到182.0和160.0pg/mL;吸附等温线显示,以赖氨酸为配基的吸附剂其吸附量随溶液内毒素浓度增加而线性增加,符合Langmuir吸附方程,吸附能力强,具有一定的临床应用前景.

关键词: 内毒素, 血液灌流, 球形纤维素, 氨基酸

Abstract: Bacterial endotoxins(ET) are recognized as the principal mediators in endotoxin shock and multiple organ failure(MOF). Removal of ET in blood by hemoperfusion is one of the effective methods to remove the pathogenic toxin. In the present study,8 amino acids and a poly-L-Lys immobilized on cellulose beads were used in static adsorption experiments to adsorb ET in water solution. Adsorption capacity,adsorption isotherm and affinity mechanism of immobilized amino acid on adsorbents were investigated. The experimental results show that the ligands,Arg and Lys have the highest adsorption capacity which are 182.0 and 160.0 pg/mL gel in 100.0 pg/mL ET solution respectively. Adsorption isotherm of Lys ligand shows that the adsorption amounts of ET increased linearly with increased ET concentrations which shows a good adsorption capacity. The Lys immobilized adsorbent showed to have a high potential for clinical application in endotoxin removal from patients.

Key words: Endotoxin, Hemoperfusion, Cellulose bead, Amino acid

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