高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (5): 982.

• 研究快报 • 上一篇    下一篇

量子点荧光标记在重组噬菌体表面展示肽与胰岛素受体相互作用中的应用

李鸿梅1, 刘含智1, 张皓2, 朱贵宾3, 王丽萍1, 杨柏2, 李惟1   

  1. 1. 吉林大学生命科学学院;
    2. 吉林大学化学学院, 教育部超分子结构与材料开放实验室;
    3. 吉林大学基础医学院, 长春 130023
  • 收稿日期:2003-10-23 出版日期:2004-05-24 发布日期:2004-05-24
  • 通讯作者: 王丽萍(1967年出生),女,博士,副教授,主要从事分子识别的研究.E-mail:lipingwang67@sina.com E-mail:lipingwang67@sina.com
  • 基金资助:

    国家自然科学基金(批准号:29774010)资助

Application of Quantum Dots Labeling to the Interaction Between Reconstructed-phage Display Peptide and Insulin Receptor

LI Hong-Mei1, LIU Han-Zhi1, ZHANG Hao2, ZHU Gui-Bin3, WANG Li-Ping1, YANG Bai2, LI Wei1   

  1. 1. College of Life Science, Jilin University;
    2. College of Chemistry, Key Lab of Supramolecular Structure and Materials of Ministry of Educational, Jilin University;
    3. School of Basic Medical Sciences, Jilin University, Changchun 130023, China
  • Received:2003-10-23 Online:2004-05-24 Published:2004-05-24

关键词: 量子点, 重组噬菌体, 荧光标记

Abstract: Luminescent quantum dots(QDs) are a promising alternative to organic dyes for fluorescence-based applications. Filamentous phage was labeled with QDs and its infective capability is observed after labeling. The experimental result shows that the labeling phage can still infect E.coli K91 normally. We have also developed the procedures for using QDs to label CHO-IR cells and Mouse Kidney tissue slice. The tissue slice remained stably labeled for over 10 d and the cells remained stably labeled even for over 5 months.

Key words: Quantum dot, Reconstructed-phage, Fluorescence label

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