高等学校化学学报

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (2): 243.

• 研究论文 • 上一篇    下一篇

环丙沙星铽荧光配合物与DNA的相互作用及染色新方法

杨军1, 任宇2, 徐怡庄1, 苏允兰1, 张莉1, 刘智1, 王凡1, 杨丽敏1, 王晶1,3, 高宏成1, 叶放4, 吴瑾光1   

  1. 1. 北京大学化学与分子工程学院, 稀土材料化学及应用国家重点实验室, 北京 100871;
    2. 西安交通大学第一附属医院肿瘤外科系, 西安 710049;
    3. 北京大学口腔医学院, 北京 100871;
    4. 广州白云山制药有限公司, 广州 510515
  • 收稿日期:2003-01-27 出版日期:2004-02-24 发布日期:2004-02-24
  • 通讯作者: 吴瑾光(1934年出生),女,教授,博士生导师,从事无机化学研究.E-mail;w jg@chem.pku.edu.cn E-mail:w jg@chem.pku.edu.cn
  • 基金资助:

    国家重点基础研究发展规划项目(批准号;G1998061307,2002CCAO1900);国家自然科学基金(批准号;20023005,50203001)资助

Interaction Between DNA and Terbium(Ⅲ)-Ciprofloxacin Fluorescence Complex and New Tissue Staining Method

YANG Jun1, REN Yu2, XU Yi-Zhuang1, SU Yun-Lan1, ZHANG Li1, LIU Zhi1, WANG Fan1, YANG Li-Min1, WANG Jing1,3, GAO Hong-Cheng1, YE Fang4, WU Jin-Guang1   

  1. 1. College of Chemical and Molecular Engineering, State Key Laboratory of Rare Earth Material Chemistry and Application, Peking University, Beijing 100871, China;
    2. Department of Oncology Surgery, the First Hospital of Xi′an Jiaotong University, Xi′an 710049, China;
    3. Collge of Stomatology Medicine, Peking University, Beijing 100871, China;
    4. Guangzhou Baiyunshan Pharmaceutical Limited Company, Guangzhou 510515, China
  • Received:2003-01-27 Online:2004-02-24 Published:2004-02-24

PDF (PC)

被引次数

33

摘要： 合成了环丙沙星铽荧光配合物,并研究其与DNA分子的相互作用.对比DNA分子吸附配合物分子前后的显微红外及荧光光谱发现,以环丙沙星分子为配体的稀土配合物同DNA间存在相互作用,这种作用诱导了DNA二级结构的构象变化.基于这种相互作用,研究了环丙沙星铽配合物对口腔粘膜组织切片的荧光染色,得到了同经典的苏木精-伊红染色法类似的反差效果.

关键词: 稀土配合物, 环丙沙星, 喹诺酮, 荧光, 组织染色

Abstract: A new lanthanide complex that gives off green fluorescence upon exciting by UV radiation was synthesized by using terbium chloride and ciprofloxacin. The complex binds firmly on DNA and induces conformation changes of DNA based on the analysis of FTIR and fluorescence spectra. Strong fluorescence from terbium ions can be observed from the resultant composite under UV light. Thus, it is possible that the terbium ciprofloxacin complex can be developed as a fluorescent dye in biological and pathological studies. The results indicate that the complex is concentrated on the nuclei regions in the sections of oral tissues and the nuclei are highlighted under fluorescent microscope.

Key words: Rare-earth complex, Ciprofloxacin, Quinolone, Fluorescence, Tissue staining

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