高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (11): 2034-2037.

• 研究论文 • 上一篇    下一篇

二色补血草多糖的结构表征及其对Hela细胞的抑制作用

张连茹1, 陈喀林2, 李妮1, 邹国林1   

  1. 1. 武汉大学生命科学学院, 武汉430072;
    2. 内蒙古赤峰学院实验中心, 赤峰024001
  • 收稿日期:2003-09-09 出版日期:2004-11-24 发布日期:2004-11-24
  • 基金资助:

    国家自然科学基金(批准号:30370366)资助

Isolation and Structural Analysis of the Polysaccharides of Limonium bicolor and the Inhibition to Hela Cell

ZHANG Lian-Ru1, CHEN Ka-Lin2, LI Ni1, ZOU Guo-Lin1   

  1. 1. College of Life Sciences, Wuhan University, Wuhan 430072, China;
    2. Experimental Center, Chifeng College, Chifeng 024001, China
  • Received:2003-09-09 Online:2004-11-24 Published:2004-11-24

摘要: 从中药二色补血草中分离提取到一种水溶性多糖(LP).1HNMR,13CNMR,IR,HPLC和GC-MS等方法分析表明,LP是以α-D-[GlcA(1→6)Glc]二糖为结构单位,每个重复的二糖单位彼此以α-(1→4)糖苷键连接成直链多糖.荧光光谱显示LP的荧光谱峰出现在460nm附近,Zn2+,Ca2+通过与LP络合,其荧光强度增加.MTT实验表明,LP具有抑制肿瘤细胞活性,对Hela细胞抑制的IC50为62.2μg/mL.

关键词: 二色补血草, 多糖, 结构, Hela细胞, IC50

Abstract: Soluble polysaccharides were isolated from Limonium bicolor Kunge(Bge.) and purified by conventional method. IR, GC-MS, 13C NMR and 1H NMR were used to analyze the structures of the polysaccharides. The result showed that glucose and glucose acid were the main components of the polysaccharides, and the bond between GlcA and Glc was α-(1→6), then the disaccharide unit was connected by α-(1→4) linkage. The polysaccharides were fluorescent with the biggest emission peak at 460 nm, and Zn2+ or Ca2+ enhanced the fluorescence intensity of LP. Much more research proved that LP had the function of inhibiting the growth of Hela cells, with the IC50 being 62.2 μg/mL.

Key words: Limonium bicolor, Polysaccharides, Structure, Hela cells, IC50

中图分类号: