高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (5): 798.

• 论文 • 上一篇    下一篇

溶胶-凝胶法固定胆固醇氧化酶偶联普鲁士蓝化学修饰电极的研究

李建平1,2, 彭图治1   

  1. 1. 浙江大学西溪校区化学系, 杭州 310028;
    2. 桂林工学院应用化学系, 桂林 541004
  • 收稿日期:2001-12-06 出版日期:2003-05-24 发布日期:2003-05-24
  • 通讯作者: 彭图治(1946年出生),男,教授,博士生导师,主要从事电分析化学和生物分析化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29975024);浙江省自然科学基金;浙江省分析测试基金

Cholesterol Biosensors Based on Cholesterol Oxidase Immobilized in a Silica Sol-gel Matrix on a Prussian Blue Modified Electrode

LI Jian-Ping1,2, PENG Tu-Zhi1   

  1. 1. Department of Chemistry, Xixi Campus, Zhe Jiang University, Hangzhou 310028 China;
    2. Department of Applied Chemistry, Guilin Institute of Technology, Guilin 541004, China
  • Received:2001-12-06 Online:2003-05-24 Published:2003-05-24

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被引次数

38

摘要： 用溶胶-凝胶法将胆固醇氧化酶固定在普鲁士蓝修饰的玻碳电极表面,制成了一种新型胆固醇传感器,实现了低电位下对胆固醇的间接测定,胆固醇的测定范围伏安法为5×10-7～8×10-5mol/L,安培法为5×10-6～5×10-4mol/L.伏安法检出限为1.2×10-7mol/L,是目前所见灵敏度最高的胆固醇传感器之一,该传感器对胆固醇的测定可避免常规电化学传感器测定中由于样品中大量存在的易氧化物质所带来的干扰,该传感器的寿命长,使用次数在300次以上.

关键词: 生物传感器, 胆固醇氧化酶, 普鲁士蓝, 溶胶-凝胶, 电极

Abstract: Acholesterol biosensor prepared by immobilization of cholesterol oxidase in a silica sol-gel matrix on the surface of Prussian Blue-modified glass carbon electrode was reported. The properties of the sensor were investigated. It is based on the detection of hydrogen peroxide produced by ChOx at 0. 0-0. 1 V. The measurements conditions were optimized. Cholesterol can be determined in the concentration range of 5 × 10-7～8 × 10-5mol/Lby voltammetry or 5 ×10-6～5 × 10-4mol/Lby amperometry. The method gives a high sensitivity owing to the electrocatalytic layer of Prussian Blue to the electro-reduction of enzymatic production of hydrogen peroxide. In addition, the effect of easily oxidizable substances presented in samples such as ascorbic acid and uric acid can be minimized.

Key words: Biosensor, Cholesterol oxidase, Prussian Blue, Sol-gel, Electrode

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