高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (4): 612.

• 研究简报 • 上一篇    下一篇

功能性CdS纳米荧光探针荧光增敏法测定人血清白蛋白

汪乐余, 周运友, 朱昌青, 张明翠, 陶海升, 王伦   

  1. 安徽师范大学化学与材料科学学院, 芜湖 241000
  • 收稿日期:2001-11-19 出版日期:2003-04-24 发布日期:2003-04-24
  • 通讯作者: 王E-mai伦(1964年出生),男,硕士,教授,从事光谱分析研究.E-mail:wangly73@yahoo.com E-mail:wangly73@yahoo.com
  • 基金资助:

    国家教育部高等学校骨干教师资助项目(批准号:99JL079)资助

Fluorescence Enhancing Method for the Determination of Human Serum Albumin with Functionalized CdS Nanoparticles as Fluorescence Probes

WANG Le Yu, ZHOU Yun You, ZHU Chang Qing, ZHANG Ming Cui, TAO Hai Sheng, WANG Lun   

  1. College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, China
  • Received:2001-11-19 Online:2003-04-24 Published:2003-04-24

关键词: CdS纳米粒子, 荧光探针, 人血清白蛋白

Abstract: CdS nanoparticles were prepared and modified with mercaptoacetic acid. The functionalized nanoparticles were water soluble and biocompatible and used as fluorescence probe in the determination of Human serum albumin(HSA) and γ globulin(γ G). Under the optimal conditions, linear relationships were found between the enhanced intensity of fluorescence and the concentration of protein in the range of 0.2 and 3.5 μg/mL for HSA and 0.2 and 11.0 μg/mL for γ G. The limits of detection were 0.09 μg/mL for HSA and 0.18 μg/mL for γ G.

Key words: CdS Nanoparticle, Fluorescence probe, Human serum albumin

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