高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (12): 2480-2485.doi: 10.7503/cjcu20190366

• 分析化学 • 上一篇    下一篇

可视化检测次氯酸的苯并咪唑类荧光增强型探针

张勇(),申城,幸志荣,陈归柒,卢资,侯志兵,陈雪梅   

  1. 湖北理工学院化学与化工学院, 矿区环境污染控制与修复湖北省重点实验室, 黄石 435003
  • 收稿日期:2019-07-01 出版日期:2019-12-04 发布日期:2019-12-04
  • 通讯作者: 张勇 E-mail:zy0340907@163.com
  • 基金资助:
    国家自然科学基金(51173060);矿区环境污染控制与修复湖北省重点实验室开放基金(2017108);湖北理工学院校级重点项目(15xjz01A)

Benzimidazole-Derived Fluorescence Enhancement Probe for Visual Detection of HClO

Yong ZHANG(),Cheng SHEN,Zhirong XING,Guiqi CHEN,Zi LU,Zhibing HOU,Xuemei CHEN   

  1. College of Chemistry and Chemical Engineering, Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Hubei Polytechnic University, Huangshi 435003, China
  • Received:2019-07-01 Online:2019-12-04 Published:2019-12-04
  • Contact: Yong ZHANG E-mail:zy0340907@163.com
  • Supported by:
    ? Supported by the National Natural Science Foundation of China(51173060);the Opening Foundation of Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, China(2017108);the Key Program of Hubei Polytechnic University, China(15xjz01A)

摘要:

合成了一种新型的苯并咪唑类荧光增强型探针2-{[4-(1H-苯并咪唑-2-基)苯亚甲基]氨基}-3-氨基马来腈(ZY12), 用光谱方法研究了其对次氯酸的识别作用. 实验结果表明, ZY12对ClO -表现出较高的选择性和灵敏度, 且响应快速, 抗干扰能力强; 将ClO -加入到ZY12溶液中后, 反应体系的荧光强度随ClO -浓度的增加而逐渐增强, 并伴随着荧光颜色由微蓝色到亮蓝色的变化, 可实现对ClO -的可视化检测; 在1.8×10 -6~3.4×10 -5 mol/L浓度范围内, ZY12能够定量检测ClO -, 检测限为2.8×10 -7 mol/L; ZY12可应用于不同水样中ClO -的检测, 并能对HeLa活细胞中的ClO -进行荧光成像.

关键词: 次氯酸, 荧光探针, 可视化检测, 荧光成像

Abstract:

A novel benzimidazole-derived fluorescence enhancement probe 2-{[4-(1H-benzo[d]imidazol-2-yl)benzylidene]amino}-3-aminoma-leonitrile(ZY12) was synthesized, and its recognition of HClO was researched with spectral methods. The results showed that ZY12 demonstrated relatively high selectivity and sensitivity to ClO -. It had quick response and strong anti-interference ability. Upon addition of ClO - to ZY12 solution, the fluorescence intensity of reaction system increased with the increasing concentration of ClO -, accompanied with fluorescence color change from faint blue to bright blue, realizing the visual detection of ClO -. ZY12 could quantitatively detect ClO - at concentrations ranging from 1.8×10 -6—3.4×10 -5 mol/L with a detection limit of 2.8×10 -7 mol/L. ZY12 was used to determine ClO - in various water samples, and it could also be applied to intracelluar ClO - imaging in living HeLa cells.

Key words: Hypochlorous acid, Fluorescent probe, Visual detection, Fluorescence imaging

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