高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (12): 2486-2493.doi: 10.7503/cjcu20190354

• 分析化学 • 上一篇    下一篇

Cr 3+比色荧光探针的合成及细胞成像应用

张莹莹,黄译文,赵冰,王丽艳,宋波()   

  1. 齐齐哈尔大学化学与化学工程学院, 齐齐哈尔 161006
  • 收稿日期:2019-06-24 出版日期:2019-12-04 发布日期:2019-12-04
  • 通讯作者: 宋波 E-mail:songboliu@sina.com
  • 基金资助:
    黑龙江省科学基金留学归国人员科学基金(LC2017004);黑龙江省教育厅基本科研业务专项(135209209);黑龙江省自然科学基金联合引导项目(LH2019B021)

Synthesis of a Colorimetric Fluorescent Probe of Cr 3+ and Its Application in Cell Imaging

Yingying ZHANG,Yiwen HUANG,Bing ZHAO,Liyan WANG,Bo SONG()   

  1. College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar 161006, China
  • Received:2019-06-24 Online:2019-12-04 Published:2019-12-04
  • Contact: Bo SONG E-mail:songboliu@sina.com
  • Supported by:
    ? Supported by the Science Foundation for Returnees of Heilongjiang Province, China(LC2017004);the Education Department Basic Research Business Special Foundation of Heilongjiang Province, China(135209209);the Natural Science Foundation of Heilongjiang Province, China(LH2019B021)

摘要:

以2,3,3-三甲基-3H-苯并[e]吲哚和对二甲氨基苯甲醛为原料, 乙醇作溶剂, 在酸性催化条件下, 通过一步反应合成了比色荧光探针B. 在EtOH/HEPES(pH=7.4)体积比为9∶1的混合体系中, 向探针B溶液中加入Cr 3+后, 溶液颜色由淡黄色变为紫红色, 说明探针B可以对Cr 3+进行裸眼识别. 紫外-可见吸收光谱和荧光发射光谱分析表明, 探针B对Cr 3+的选择性好、 灵敏度高且对EDTA有良好的接力识别. 探针B对Cr 3+的结合常数Ka=0.28×10 2 mol/L, 检出限为1.90×10 -8 mol/L, 该检出限低于世界卫生组织(WHO)规定的饮用水中Cr 3+的最大含量(9.60×10 -8 mol/L). 利用荧光发射光谱对实际水样中Cr 3+的浓度进行了定量检测. 探针B也可应用于对活细胞中Cr 3+的检测, 具有较好的应用前景和实用价值.

关键词: 苯并吲哚, 荧光探针, Cr 3+, 水样, 细胞

Abstract:

Colorimetric fluorescence probe B was synthesized from 2,3,3-trimethyl-3H-benzo[e]indole and p-dimethylaminobenzaldehyde using acidic solvent as catalyst and ethanol as solvent. In the mixed system(pH=7.4) of V(EtOH)∶V(HEPES)=9∶1, the color of solution B changed from pale yellow to purple-red after added Cr 3+ to probe B solution, indicating that probe B can recognize Cr 3+ with naked-eye. Fluorescence emission spectra shows that probe B had good selectivity of Cr 3+, high sensitivity and good recognition ability with EDTA. The binding constant(Ka) of probe B on Cr 3+ was 0.28×10 2 mol/L and the detection limit was 1.90×10 -8 mol/L. The detection limit was lower than the maximum Cr 3+ content(9.60×10 -8 mol/L) in drinking water stipulated by WHO. In addition, the concentration of Cr 3+in actual water samples was quantitatively detected by fluorescence emission spectra. Probe B has been applied to the detection of Cr 3+ in living cells, which has a good application prospect and practical value.

Key words: Benzindole, Fluorescence probe, Cr 3+, Water sample, Cell

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