高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (4): 609.

• 研究快报 • 上一篇    下一篇

利用水相合成的量子点标记木瓜蛋白酶的研究

林章碧1, 张皓2, 陈奇丹1, 万异1, 杨柏2, 苏星光1,3, 张家骅3, 金钦汉1   

  1. 1. 吉林大学化学学院分析科学研究所;
    2. 吉林大学超分子结构与材料教育部重点实验室, 长春 130023;
    3. 中国科学院激发态物理开放实验室, 长春 130021
  • 收稿日期:2002-11-25 出版日期:2003-04-24 发布日期:2003-04-24
  • 通讯作者: 苏星光(1966年出生),女,博士,副教授,从事光谱分析方面的研究.E-mail:suxg@jlu.edu.cn E-mail:suxg@jlu.edu.cn
  • 基金资助:

    国家自然科学基金(批准号:20075009)资助

Studies on Labeling of Papain with Quantum DotsSynthesized in Aqueous Solution

LIN Zhang Bi1, ZHANG Hao2, CHEN Qi Dan1, WAN Yi1, YANG Bai2, SU Xing Guang1,3, ZHANG Jia Hua3, JIN Qin Han1   

  1. 1. Institute of Analytical Science, College of Chemistry;
    2. Key Lab for Supramolecular Structure and Materials, Ministry of Education, Jilin University, Changchun 130023, China;
    3. Laboratory of Excited State Processes, Chinese Academy of Sciences, Changchun 130021, China
  • Received:2002-11-25 Online:2003-04-24 Published:2003-04-24

PDF (PC)

被引次数

113

关键词: 量子点, 木瓜蛋白酶, 静电相互作用

Abstract: The feasibility of the mercaptopropyl acid modified CdTe nanoparticles synthesized in aqueous solution to the labeling of biomoleculespapain via electrostatic interaction was studied in this paper. The absorption spectrum of CdTe papain solution became more flat than that of CdTe nanoparticles in the wavelength range from 400 to 600 nm. While the emission peak of CdTe papain solution underwent a red shift, the intrinsic emission band width was unchanged. The TEM images of monodisperse mercaptopropyl acid stabilized CdTe nanoparticles and CdTe papain solution were compared and the effect of pH of buffer solution was studied. It was proved that the red shift of the emission peak was the result of the electrostatic interaction between CdTe nanoparticles and papain.

Key words: Quantum dots, Papain, Electrostatic interaction

中图分类号: 

TrendMD: