高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (12): 2268.

• 论文 • 上一篇    下一篇

金电极表面聚赖氨酸固定微过氧化物酶-11的电化学研究

王凤彬1, 郑军伟2, 李晓伟1, 季媛2, 高颖3, 邢巍1, 陆天虹1   

  1. 1. 中国科学院长春应用化学研究所电分析化学国家重点实验室, 长春 130022;
    2. 苏州大学化学系, 苏州 215006;
    3. 哈尔滨师范大学化学系, 哈尔滨 150080
  • 收稿日期:2002-08-08 出版日期:2003-12-24 发布日期:2003-12-24
  • 通讯作者: 陆天虹(1943年出生),男,博士,研究员,博士生导师,主要从事生物电化学和化学电源的研究.E-mail:tianhonglu@263.net E-mail:tianhonglu@263.net
  • 基金资助:

    国家自然科学基金(批准号:20073028,20243022);黑龙江省自然科学基金(批准号:B2000-06)资助

Electrochemical Study of Microperoxidase-11 Immobilized on the Surface of a Poly-L-lysine Modified Gold Electrode

WAGN Feng-Bin1, ZHENG Jun-Wei2, LI Xiao-Wei1, JI Yuan2, GAO Ying3, XING Wei1, LU Tian-Hong1   

  1. 1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Department of Chemistry, Suzhou University, Suzhou 215006, China;
    3. Department of Chemistry, Haerbin Normal University, Haerbin 150080, China
  • Received:2002-08-08 Online:2003-12-24 Published:2003-12-24

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被引次数

12

摘要： 通过聚赖氨酸修饰将微过氧化物酶-11(MP-11)固定在金电极表面,制备成MP-11修饰电极.修饰在电极表面上的MP-11的血红素活性中心与电极之间可进行直接的电子传递反应,其氧化还原式电位为-0.39V.该修饰电极对氧的还原具有电催化活性.当MP-11与咪唑发生轴向配位反应时,其氧化还原式电位发生负移,此时对氧的还原不再具有电催化活性.

关键词: 微过氧化物酶-11, 聚赖氨酸, 咪唑, 电催化, 紫外-可见吸收光谱

Abstract: MP-11 was immobilized on the surface of the poly-L-lysine modified gold electrode. Direct electron transfer between the heme and the electrode was observed. The formal redox potential for MP-11 immobilized at the gold electrode is -0.39 Vin the phosphate buffer solution(pH=7.0). The MP-11 modified electrode possesses the good electrocatalytic activity for the reduction of O2When imidazole becomes the sixth ligand, the formal redox potential of MP-11 would shift to the negative direction and the electrocatalytic activity of MP-11 for the reduction of O2is significantly inhibited.

Key words: Microperoxidase-11, Poly-L-lysine, Imidazole, Electrocatalysis, UV-Vis absorption spectrum

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