高等学校化学学报

高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (11): 1967.

• 论文 • 上一篇    下一篇

电中性分子间相互作用分析的毛细管电泳方法研究

夏之宁, 刘毅, 郭宝元, 陈国华   

  1. 重庆大学化学化工学院, 重庆 400044
  • 收稿日期:2002-11-07 出版日期:2003-11-24 发布日期:2003-11-24
  • 通讯作者: 夏之宁(1961年出生),男,博士,教授,从事分析化学/药物化学研究.E-mail:zhnxia@yahoo.com.cn E-mail:zhnxia@yahoo.com.cn
  • 基金资助:

    国家自然科学基金(批准号:20175038);教育部跨世纪优秀人才培养计划资助;教育部高等学校青年教师奖(批准号:教人司[2002]123)资助

Studies on the Interaction Analysis of Neutral Molecules by Using Capillary Electrophoresis

XIA Zhi-Ning, LIU Yi, GUO Bao-Yuan, CHENGuo-Hua   

  1. Institute of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China
  • Received:2002-11-07 Online:2003-11-24 Published:2003-11-24

PDF (PC)

被引次数

12

摘要： 使用具有负有效淌度的十二烷基磺酸钠胶束赋予中性分子有效淌度,以中性主体分子18-冠-6和中性客体分子对-硝基苯胺形成中性超分子包结化合物为实例,建立了中性分子相互作用的毛细管电泳分析方法,发现对-硝基苯胺和18-冠-6与SDS的相互作用存在相当的差异.测得18-冠-6和对-硝基苯胺的lgK为4.14,18-冠-6与2-氨基吡啶、18-冠-6与SDS以及SDS与2-氨基吡啶的lgK分别为3.65,3.13和1.15.

关键词: 相互作用, 超分子, 毛细管电泳, 18-冠-6, 中性分子

Abstract: Because neutral molecules have no absolute mobilities, the capillary electrophoresis utilizing peak-shifting experiment can not be used to analyze the interaction of them.In this paper, the neutral molecules have been imparted apparent mobilities by sodium dodecyl sulfate(SDS) micelle, which has negative charge and negative apparent mobilities.The 18-crown-6 and p-nitroaniline without charge under a controlled condition were used as the samples of neutral molecules, and they were able to forMA neutral supramolecule by interaction.Through the research, an interaction analytical method by using capillary electrophoresiSIs set up.The logarithm of binding constant of 18-crown-6 with p-nitroaniline was obtained to be 4.14.In addition, comparative differences of hydrophobicity between 18-crown-6 and p-nitroaniline to SDS was found, respectively.Further more, for multiple interactions, the interaction analytical method of supramolecules has also been set up, which has more accuracy and availability.The logarithms of binding constant of 18-crown-6 to 2-aminopyridine and SDS, and 2-aminopyridine to SDS were obtained to be 3.65, 3.13 and 1.15, respectively.After the development of the peak-shifting was applied to the neutral molecules, the principles, available experimental conditions, and assumptionSIn the measurement have been discussed.This approach in this paper could be developed as an interaction analysis method of multiple componentSIn a complex system.

Key words: Interaction, Supramolecule, Capillary electrophoresis, 18-Crown-6, Neutral molecule

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