高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (8): 1524.

• 研究论文 • 上一篇    下一篇

人血清脂蛋白与胆固醇修饰的葡聚糖的相互作用

曹宁宁1, 俞耀庭2, 王满燕2, 王连永2, 陈长治2   

  1. 1. 天津理工学院生物与化学工程系;
    2. 南开大学分子生物研究所教育部生物活性材料重点实验室, 天津 300071
  • 收稿日期:2001-04-24 出版日期:2002-08-24 发布日期:2002-08-24
  • 通讯作者: yaoting@public1.tpt.tj.cn
  • 作者简介:俞耀庭(1932年出生),男,教授,博士生导师,主要从事生物化学和生物活性材料研究.E-mail:yaoting@public1.tpt.tj.cn
  • 基金资助:

    国家重点基础研究与发展基金(批准号:9964707);天津市高等学校科技发展基金项目(批准号:20010404)资助

The Interaction of Human Lipoprotein with Cholesterol Modified Dextran in Serum

CAO Ning-Ning1, YU Yao-Ting2, WANG Man-Yan2, WANG Lian-Yong2, CHEN Chang-Zhi2   

  1. 1. Chemistry and Engineering Department, Tianjin Science and Technology University, Tianjin 300191, China;
    2. Bioactive Materials Research Laboratory, Nankai University, Tianjin 300071, China
  • Received:2001-04-24 Online:2002-08-24 Published:2002-08-24

PDF (PC)

被引次数

10

摘要： 采用浊度法和高效液相色谱法研究了人血清低密度脂蛋白(LDL)和极低密度脂蛋白(VLDL)与胆固醇修饰的葡聚糖(CHD)的相互作用.浊度法研究结果表明,CHD与VLDL混合溶液的浊度大于CHD与LDL混合溶液的浊度.CHD浓度和CHD链上胆固醇含量都对溶液的浊度产生影响.当CHD浓度为0.3mg/mL,CHD链上胆固醇含量为4个/100个糖环时,混合溶液的浊度可达到最大值,表明LDL或VLDL聚集的程度最大.Ca2+对CHD与LDL或VLDL的相互作用没有影响.高效液相色谱的研究结果表明,CHD与LDL的结合使得复合物分子量增加;CHD分子量增大,复合物分子量也增加;当葡聚糖上胆固醇含量为4.0时,复合物分子量最大.实验结果表明,CHD能引起LDL或VLDL的自聚集.

关键词: 相互作用, 低密度脂蛋白, 极低密度脂蛋白, 胆固醇修饰的葡聚糖

Abstract: The interaction of human low-density lipoprotein(LDL) and very-low-density lipoprotein(VLDL) with cholesterol modified dextran(CHD) in serum was studied by turbidity and high performance liquid chromatography(HPLC)method. The solution turbidity of VLDLreacting with CHDwas higher than that of LDLwith CHDand was influenced by the concentration of CHD, cholesterol content of CHDand molecular weight of CHD. The turbidity reached the maximum value when the concentration of CHDwas 0.3 mg/mLand cholesterol content was 4 cholesterol group/100 dextran glucose units. Ca 2+ had no influence on the interaction of VLDLand LDLwith CHD.The molecular weights of LDL CHDcomplex were monitored by HPLCwhich showed that CHDmolecules could associate multi molecules of LDLcorrelating with the results of turbidity experiments. The HPLCexperiments showed that when cholesterol substitution number was 4/100 glucose units, the interaction of LDLwith CHDreached the optimal condition and the complexation molecular weight increased with increasing dextran molecular weight. The experimental results show that CHDcan induce self-aggregation of LDLor VLDL.

Key words: Interaction, Low density lipoprotein, Very low-density lipoprotein, Cholesterol modified dextran

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