高等学校化学学报

高等学校化学学报 ›› 2002, Vol. 23 ›› Issue (7): 1429.

• 研究论文 • 上一篇    下一篇

N-去甲万古霉素的亲和吸附

赵庆香, 阎虎生, 程晓辉, 何炳林   

  1. 南开大学高分子化学研究所, 吸附与分离功能高分子材料国家重点实验室, 天津 300071
  • 收稿日期:2001-03-09 出版日期:2002-07-24 发布日期:2002-07-24
  • 通讯作者: 阎虎生(1959年出生),男,教授,博士生导师,从事功能高分子和多肽合成研究.E-mail:yanhs@public.tpt.tj.cn E-mail:yanhs@public.tpt.tj.cn
  • 基金资助:

    教育部重点科技基金(批准号:98098);吸附与分离功能高分子材料国家重点实验室开放基金(批准号:kf025)

Affinity Adsorbents for N-Demethylvancomycin

ZHAO Qing-Xiang, YAN Hu-Sheng, CHENG Xiao-Hui, HE Bing-Lin   

  1. Institute of Polymer Chemistry, State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Nankai University, Tianjin 300071, China
  • Received:2001-03-09 Online:2002-07-24 Published:2002-07-24

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摘要： 通过交联聚丙烯酸甲酯与乙醇胺反应,形成聚(N-羟乙基丙烯酰胺)树脂,在酸催化作用下与环氧氯丙烷反应,形成含有α-羟基氯乙基的树脂.含α-羟基氯乙基的树脂与D-丙氨酸、L-丙氨酸或甘氨酸反应,分别得到含有这3种氨基酸的吸附剂.这3种吸附剂吸附N-去甲万古霉素的结果表明,含D-丙氨酸的吸附剂的吸附量最大,含甘氨酸的吸附剂的吸附量次之,而含L-丙氨酸的吸附剂不吸附N-去万古霉素.说明前两种吸附剂对N-去甲万古霉素存在亲和吸附作用.含D-丙氨酸吸附剂的最佳吸附pH值为5.8,当吸附液中的盐(NaCl)浓度增加时,吸附量降低.用0.4mol/LNa2CO3/CH3CN(摩尔比7∶3,pH=9.5)作为洗脱剂可完全脱附被吸附的N-去甲万古霉素.

关键词: 亲和, 吸附, 万古霉素, N-去甲万古霉素, 聚合物

Abstract: Poly( N-hydroxyethyl acrylamide) resin was prepared by reacting ethanolamine with crosslinked poly(methyl acrylate). α Hydroxyl chloroethyl groups were introduced into the resin by treating the resin with epichlorohydrin with the acid catalysis. Three adsorbents with D-alanine, Lananine or glycine, respectively, were made by reacting the α hydroxyl chloroethyl group containing resin with the corresponding amino acids. The results of adsorbing N-demethylvan-comycin of the adsorbents indicated that the adsorbent with D-alanine had the highest adsorption capacity, the second was the adsorbent with glycine, while the adsorbent with Lalanine didn't adsorb N-demethylvan-comycin. The optimal pHfor the adsorption for adsorbent with D-alanine was ～5.8 and the adsorption capacity decreased with increasing salt(NaCl) concenttration. The adsorbed N-demethylvan-comycin was completely desorbed with 0.4 mmol/L Na 2CO3/CH3CN(molar ratio 7∶3, pH=9.5).

Key words: Affinity, Adsorption, Vancomycin, N Demethylvancomycin, Polymer

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