高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (9): 1486.

• 研究论文 • 上一篇    下一篇

紫细菌反应中心色素蛋白复合膜电子转移研究

赵杰权1, 邹永龙2, 刘宝红1, 彭雪松1, 徐春和2, 孔继烈1   

  1. 1. 复旦大学化学系, 上海 200433;
    2. 中国科学院上海植物生理研究所, 上海 200032
  • 收稿日期:2000-05-24 出版日期:2001-09-24 发布日期:2001-09-24
  • 通讯作者: 孔继烈(1964年出生),男,博士,教授,从事生物分子光电化学、发光/变色材料研究.
  • 基金资助:

    国家自然科学基金(批准号:39970195;29905001;39730040);国家科委重点基金(批准号:G1998010100)资助

Studies on the Electron Transfer for Reaction Center Isolated from Rhodobacter Sphaeroides in Self-assembly Composite Film

ZHAO Jie-Quan1, ZOU Yong-Long2, LIU Bao-Hong-PENG Xue-Song1, XU Chun-He1, KONG Ji-Lie2, CUI Da-Fu1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan 430072, China;
    2. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 100080, China
  • Received:2000-05-24 Online:2001-09-24 Published:2001-09-24

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被引次数

1

摘要： 采用自组装单层膜方法在金电极表面形成单分子层的2,3-双巯基丁二酸(DMSA)、聚二甲基二丙烯氯化铵(PDDA)及紫细菌(Rhodobactersphaeroides)反应中心色素蛋白复合体的有序复合膜.使用方波伏安法研究了该蛋白复合膜的电化学行为,成功地检测到该蛋白内多电对的可逆或准可逆电子转移过程,探讨了紫细菌反应中心蛋白内各电子受体的氧化还原电位及外加电位驱动对各受体电位的影响.同时,通过对方波信号的非线形拟合,获得了该蛋白复合膜内主要电对的电子转移速率常数及电子迁移系数等相关参数.

关键词: 电子传递, 电化学, 紫细菌, 反应中心

Abstract: The composite ordered protein film was assemblied on the surface of a gold electrode by self-assembly mono layer approach using meso 2,3 dimercaptosuccinic acid(DMSA), poly dimethyldiallylammonium chloride(PDDA) and reaction center membrane protein purified from purple bacteria(Rhodobacter sphaeroides). Square wave voltammetry(SWV) was employed to study the electrochemical behavior of the protein composite film. The reversible or quasi reversible electron transfer for redox couples therein was successfully detected. The reduction potential for each redox couple and the electrochemically driven effect was also discussed. The typical SWV-results were non linearly fitted to get the kinetic parameters for the composite protein film.

Key words: Electron transfer, Electrochemistry, Rhodobacter sphaeroides, Reaction center

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