高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (3): 407.

• 研究论文 • 上一篇    下一篇

枸杞子糖缀合物LbGp4糖链的结构及其免疫活性的研究

黄琳娟1, 田庚元1, 齐春会2, 张永祥2   

  1. 1. 中国科学院上海有机化学研究所, 生命有机化学国家重点实验室, 上海 200032;
    2. 军事医学科学院毒物药物研究所, 北京 100050
  • 收稿日期:2000-02-21 出版日期:2001-03-24 发布日期:2001-03-24
  • 通讯作者: 田庚元(1940年出生),男,研究员,从事蛋白质、多糖研究.E-mail:tiangy@pub.sioc.ac.cn E-mail:tiangy@pub.sioc.ac.cn
  • 基金资助:

    国家攀登计划(批准号:970211006-4);国家自然科学基金(批准号:39600188;39730480)资助

Structure Elucidation and Immunoactivity Studies of Glycan of Glycoconjugate LbGp4 Isolated from the Fruit of Lycium barbarum L.

HUANG Lin-Juan1, TIAN Geng-Yuan1, QI Chun-Hui2, ZHANG Yong-Xiang2   

  1. 1. Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai 200032, China;
    2. Institute of Pharmacology and Toxicology Academy of Military Medical Sciences, Beijing 100050, China
  • Received:2000-02-21 Online:2001-03-24 Published:2001-03-24

PDF (PC)

被引次数

55

摘要： 枸杞子糖缀合物LbGp4经β--消除、柱层析分离后得到一条分子量高达180800的分支多糖化合物LbGp4-OL.LbGp4-OL糖基组成为n(Rha):n(Ara):n(Gal)=0.05:1.33:1.根据甲基化、部分酸水解及1HNMR、13CNMR分析,确定了其主要的结构,并对LbGp4和LbGp4-OL的免疫活性进行了研究.

关键词: 枸杞子, 糖缀合物, 结构, 免疫活性

Abstract: Glycan was isolated on Sephadex G-100 column after glycoconguate LbGp4 was released by β--elimination, then desalted on Sephadex G-25 and named LbGp4-OL. The linkage between the glycan and the core protein backbone was Olinkage. The molecular weight of LbGp4-OL was 180800. The result of its elemental analysis was C39.1%, H5.72%, N 0%. Component analysis showed that it was composed of Rha, Ara and Gal in a molar ratio of 0.05 : 1.33 :1. The structure of repeat unit of LbGp4-OL was deduced by methylation analysis, partial acid hydrolysis and 1H, 13C NMRspectroscopy of the original glycan and products of its partial hydrolysis. Methylation analysis of LbGp4-OLindicated that highly branching unit in LbGp4-OLwas →3,4)Gal(1→, the major non reducing end was Ara(1→. LbGp4-OL' was obtained by partial acid hydrolysis. Comparing the results of methylation analysis of LbGp4 with that of LbGp4-OL', we can deduced that the main chain of LbGp4-OLwas composed of →4)Gal(1→. 1H and 13C NMRspectra of LbGp4-OLand LbGp4-OL' indicated that Ara(1→ was α-furanose, →3)Ara(1→ and →5)Ara(1→ were β- furanose, all the Gal were β- pyranose, Rha(1→ was α-pyranose, →3)Arap(1→ was α-pyranose. Immunoactivity studies of LbGp4 and its glycan showed that LbGp4 and LbGp4-OLnot only enhanced the splenocyte proliferation in normal mouse, but also improved the reducing immune function in the senescense accelerated mouse. It demonstrated that LbGp4 and LbGp4-OLpossess a direct effect of splenocytl proliferation. In addition, the direct elevating action of LbGp4-OLon splencyte prolifexration was stronger than that of LbGp4. So it pointed out that the glycan play an important role in the immunopharmcological activity of glycoconjugate.

Key words: Lycium barbarum L, Glycoconjugate, Structure, Immunoactivity

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