高等学校化学学报

高等学校化学学报 ›› 2001, Vol. 22 ›› Issue (1): 34.

• 研究论文 • 上一篇    下一篇

量子点标记天花粉蛋白的研究

张春阳1, 马辉1, 丁尧1, 金雷1, 陈瓞延1, 苗琦2, 聂书明3   

  1. 1. 清华大学物理系单原子分子测控教育部重点实验室, 北京 100084;
    2. 中国科学院生物物理研究所生物大分子国家重点实验室, 北京 100101;
    3. 印第安那大学化学系, 美国
  • 收稿日期:2000-03-22 出版日期:2001-01-24 发布日期:2001-01-24
  • 通讯作者: 马　辉(1959年出生),男,博士,副教授,主要从事原子分子物理与生物物理方面的研究.
  • 基金资助:

    国家基金委海外青年学者合作基金(批准号:199928408);清华大学985基金项目资助

Studies on Quantum Dots-labeled Trichosanthin

ZHANG Chun-Yang1, MA Hui1, DING Yao1, JIN Lei1, CHEN Die-Yan1, MAO Pi2, NIE Shu-Ming3   

  1. 1. Educational Ministry Key Laboratory of Molecular and Nano Sciences, Department of Physics, Tsinghua University, Beijing 100084, China;
    2. National Laboratory of Biomacromolecular, Institute of Biophysics, Academia Sinica, Beijing 100101, China;
    3. Departmentof Chemistry of Indiana University, USA
  • Received:2000-03-22 Online:2001-01-24 Published:2001-01-24

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被引次数

179

摘要： 将半导体量子点应用于标记天花粉蛋白,研究了量子点标记天花粉蛋白的吸收光谱、荧光光谱和酶活性变化.与游离的QDs相比,QDs-TCS的吸收光谱在400～600nm范围内不发生明显变化;QDs-TCS的发射光谱发生蓝移,但发射半宽不变;标记上QDs后TCS的酶活性没有发生改变.利用双光子激发扫描荧光显微镜和激光共聚焦显微镜同时观察QDs-TCS在人绒癌细胞内的分布发现,QDs-TCS能够跨膜进入细胞,并在细胞核周围呈聚集分布.

关键词: 量子点, 天花粉蛋白, 酶活性

Abstract: Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCSvaried to a less extent with wavelength in the range of 400-600 nm. The fluorescent spectrum of QDs-TCSunderwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCSin comparison with trichosanthin. Distribution of QDs-TCSwithin human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCScould enter the cytoplasm across the membrane and aggregated around the nuleolus.

Key words: Quantum dots, Trichosanthin, Enzymatic activevity

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