高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (2): 202.

• 论文 • 上一篇    下一篇

锇-聚乙烯吲哚配合物修饰电极对肾上腺素的电催化氧化

龚毅, 叶蕾, 鞠先, 陈洪渊   

  1. 南京大学化学系, 配位化学国家重点实验室, 南京 210093
  • 收稿日期:1999-04-06 出版日期:2000-02-24 发布日期:2000-02-24
  • 通讯作者: 鞠　先(1964年出生),男,博士,教授,博士生导师,主要从事电分析化学和生化分析研究.
  • 基金资助:

    国家自然科学基金(批准号:29835110);江苏省自然科学基金(批准号:KB99030);教育部留学回国人员基金资助

Electrocatalysis of Osmium Poly-vinylindole Complex Modified Glassy Carbon Electrodes to Epinephrine Oxidation

GONG Yi, YE Lei, JU Huang-Xian, CHEN Hong-Yuan   

  1. Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China
  • Received:1999-04-06 Online:2000-02-24 Published:2000-02-24

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被引次数

63

摘要： 研究了配位聚合物锇-聚乙烯吲哚[Os(bpy)2(PVI)10Cl]Cl和Nafion双层膜修饰玻碳电极的电化学特性,该膜对肾上腺素(EP)的电化学氧化有催化作用,在通常的生理条件下(pH7.0),催化电流与EP浓度在1.0×10-6～8.6×10-5mol/L范围内呈良好的线性关系,相关系数为0.9987.Nafion膜排除了抗坏血酸(AA)的干扰,表现出较高的灵敏度、选择性及良好的稳定性.该电极可在+250mV下进行EP的安培法测定.用旋转圆盘电极对电催化过程的动力学进行了研究,催化速率常数kch为3.53×103mol-1·L·s-1.在较高EP浓度下,催化电流与浓度的关系表现出Michaelis-Menten型响应,Michaelis-Menten常数Km为1.47mmol/L.

关键词: 化学修饰电极, 玻碳电极, 锇-聚乙烯吲哚配合物, Nafion, 电催化氧化, 生物传感器

Abstract: The electrocatalytic characteristics of osmium complex polymer [Os(bpy)2(PVI)10Cl]Cl· (Os-PVI10) and Nafion dual-layer membrane modified glassy carbon electrodes were studied. The modified electrodes show an excellent electrocatalytical activity for the oxidation of epinephrine(EP). Under the normal physiological condition, the catalytic current of EPvs. its concentration has a good linear relation in the range of 1.0×10-6-8.4×10-5mol/Lwith the correlation coefficient of 0.9987. Nafion membrane makes the modified electrodes eliminate efficiently the interference of ascorbic acid(AA). The modified electrodes exhibited a good reproducibility, sensitivity, and stability. The amperometric determination of EPconcentration can be performed at +250 mV. The kinetic research was performed with a rotating disk electrode, and the electrocatalytical rate constant kch was evaluated to be 3.53×103mol-1·L·s-1. At a high EPconcentration, the relationship between the catalytic current and its concentration exhibits a Michaelis-Menten kinetic mechanism for the electrocatalytical process, and the constant Km is about 147 mmol/L.

Key words: Chemically modified electrodes, Glassy carbon electrodes, [Os(bpy)2(PVI)10Cl], Nafion, Electrocatalytic oxidation, Biosensor

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