高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (3): 383.doi: 10.7503/cjcu20160488

• 分析化学 • 上一篇    下一篇

MoS2中空纳米球的制备及在超灵敏microRNA电化学生物传感器中的应用

刘仁植(), 李晓严   

  1. 信阳学院理工学院应用生物化学技术研究所, 信阳 464000
  • 收稿日期:2016-07-08 出版日期:2017-03-10 发布日期:2017-02-23
  • 作者简介:联系人简介: 刘仁植, 女, 讲师, 主要从事界面电化学及生物电化学研究. E-mail: tizhizhu@126.com
  • 基金资助:
    河南省高等学校重点科研项目(批准号: 16B150014)和信阳学院校级重点项目(批准号: 2015zd04)资助.

Preparation of MoS2 Hollow Nanospheres and Application in Ultrasensitive Electrochemical Biosensing Platform for Micro-RNA Detection

LIU Renzhi*(), LI Xiaoyan   

  1. Application of Biochemical Technology Research Institute, College of Science and Technology, Xinyang College, Xinyang 464000, China
  • Received:2016-07-08 Online:2017-03-10 Published:2017-02-23
  • Contact: LIU Renzhi E-mail:tizhizhu@126.com

摘要:

采用模板法制备了二硫化钼中空球纳米材料, 利用扫描电子电子显微镜、 X射线衍射仪和Raman光谱仪对材料的形貌和结构进行表征. 将适配体固定在金纳米粒子和二硫化钼共同修饰的电极上构建了一种新型的微小核糖核酸(microRNA) 电化学生物传感器, 采用循环伏安、 微分脉冲伏安和电化学阻抗等技术对构筑的传感器进行表征. 结果表明, microRNA浓度在1.0×10-10 ~1.0×10-16 mol/L范围内峰电流(I)与microRNA浓度的负对数(-lgc)呈良好的线性, 目标miRNA的检出限为0.55×10-16 mol/L. 构建出的传感器具备选择性好、 灵敏度高、 稳定性强等特性, 具有广阔的应用前景.

关键词: 二硫化钼中空球, 微小核糖核酸, 电化学生物传感器, 信号放大

Abstract:

Molybdenum disulfide hollow sphere nanomaterial was synthesized through template-assisted method, the morphological structure was characterized by scanning electron microscopy(SEM), X-ray diffraction(XRD) and Raman spectroscopy. A novel microRNA electrochemical biosensor was constructed by immobilizing of aptamer on the electrode that was modified with Au nanoparticles and MoS2. The electrochemical behavior of the modified electrode was tested by cyclic voltammograms, differential pulse voltammetric and electrochemical impedance. The results indicate that the as-prepared biosensor shows a good linear relationship between the current variation and logarithm of the miRNA concentration ranging from 1.0×10-10 mol/L to 1.0×10-16 mol/L. The detection limit was estimated to be 0.055×10-16 mol/L. The constructed sensor shows good selectivity, high sensitivity, strong stability characteristics and has a broad application prospect.

Key words: Molybdenum disulfide hollowsphere, MicroRNA, Electrochemical biosensor, Signal amplification

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