基于Dy2(MoO4)3-AuNPs复合纳米材料的葡萄糖生物传感器

doi:10.7503/cjcu20160693

高等学校化学学报 ›› 2017, Vol. 38 ›› Issue (4): 554.doi: 10.7503/cjcu20160693

基于Dy₂(MoO₄)₃-AuNPs复合纳米材料的葡萄糖生物传感器

黄海平(), 岳亚锋, 徐亮, 吕连连, 胡咏梅

江西理工大学冶金与化学工程学院, 赣州 341000

收稿日期:2016-09-29 出版日期:2017-04-10 发布日期:2017-03-23
作者简介:联系人简介: 黄海平, 男, 博士, 副教授, 主要从事纳米材料制备、表征及应用研究. E-mail: sea-ping@163.com
基金资助:
国家自然科学基金(批准号: 21465013, 21005034)、中国博士后科学基金(批准号: 2014M551550)、江西省自然科学基金(批准号: 20114BAB213014)和江西理工大学清江青年英才支持计划资助

Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^†

HUANG Haiping^*(), YUE Yafeng, XU Liang, LÜ Lianlian, HU Yongmei

School of Metallurgy and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China

Received:2016-09-29 Online:2017-04-10 Published:2017-03-23
Contact: HUANG Haiping E-mail:sea-ping@163.com
Supported by:
† Supported by the National Natural Science Foundation of China(Nos 21465013, 21005034), the China Postdoctoral Science Foundation(No 2014M551550), the Natural Science Foundation of Jiangxi Province, China(No 20114BAB213014) and the Qingjiang Excellent Young Talents, Jiangxi University of Science and Technology, China

摘要/Abstract

摘要：

采用水热法合成了纳米材料钼酸镝[Dy₂(MoO₄)₃], 并制备了Dy₂(MoO₄)₃-AuNPs复合材料, 利用该复合材料固定葡萄糖氧化酶(GOD)构建了葡萄糖生物传感器. 通过透射电子显微镜(TEM)、紫外-可见光谱(UV-Vis)和能谱分析(EDS)等手段对所制备的材料进行了表征, 并利用电化学阻抗谱(EIS)和循环伏安(CV)曲线研究了该传感器的电化学性能. 结果表明, Dy₂(MoO₄)₃-AuNPs复合材料具有较好的生物相容性, 能增强固定化的GOD的生物活性, 并促进GOD在电极表面的电子传递速率; 该传感器在葡萄糖浓度为0.01~1.0 mmol/L范围内葡萄糖浓度与响应电流呈较好的线性关系, 最低检出限为3.33 μmol/L(S/N=3), 该生物传感器还具有较好的稳定性和重现性.

关键词: 水热法, 钼酸镝, 金纳米粒子, 葡萄糖氧化酶, 生物传感器

Abstract:

Nanometer material of dysprosium molybdate[Dy₂(MoO₄)₃] was hydrothermally synthsized and employed for the fabrication of glucose biosensor by immobilizing glucose oxidase(GOD) on the Dy₂(MoO₄)₃-AuNPs composite nanomaterial. Dy₂(MoO₄)₃ and Dy₂(MoO₄)₃-AuNPs were characterized by transmission electron microscopy(TEM), UV-Vis spectrometry and energy-dispersive spectrometry(EDS). The electrochemical behavior of the biosensor was studied via the electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV). The experimental results indicated that Dy₂(MoO₄)₃-AuNPs nanocomposite material presented good biocompatibility for GOD and could accelerate the electron transfer rate between the immobilized GOD and the electrode. The biosensor showed a good linear relationship in the concentration range of glucose from 0.01 mmol/L to 1.0 mmol/L with the lowest detection limit of 3.33 μmol/L(S/N=3). Additionally, the biosensor owned satisfying stability and reproducibility.

Key words: Hydrothermal method, Dysprosium molybdate, Gold nanoparticles, Glucose oxidase, Biosensor

TrendMD:

黄海平, 岳亚锋, 徐亮, 吕连连, 胡咏梅. 基于Dy₂(MoO₄)₃-AuNPs复合纳米材料的葡萄糖生物传感器. 高等学校化学学报, 2017, 38(4): 554.

HUANG Haiping, YUE Yafeng, XU Liang, LÜ Lianlian, HU Yongmei. Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^†. Chem. J. Chinese Universities, 2017, 38(4): 554.

图/表 8

Fig.1 TEM images of Dy2(MoO4)3(A) and Dy2(MoO4)3-AuNPs(B), energy spectrum of Dy2(MoO4)3(C) and UV-Vis spectra of Dy2(MoO4)3(a) and Dy2(MoO4)3-AuNPs(b)(D)

Fig.2 EIS of Dy2(MoO4)3-AuNPs/GOD/CS(a), Dy2(MoO4)3/GOD/CS(b) and GOD/CS(c) modified GCE electrodes in 0.1 mol/L KCl + 2 mmol/L [Fe(CN)6]3-/4-EIS frequency range: 0.1—1.0 × 105 Hz.

Fig.3 Cyclic voltammograms of Dy2(MoO4)3/CS(a), Dy2(MoO4)3-AuNPs/CS(b), GOD/CS(c), Dy2(MoO4)3/GOD/CS(d) and Dy2(MoO4)3-AuNPs/GOD/CS(e) modified GCE electrode in 0.1 mol/L N2-saturated PBS(pH=7.0) at the scan rate of 100 mV/s

Fig.4 Cyclic voltammograms of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE at different scant rates in 0.1 mol/L N2-saturated PBS(pH=7.0)(A) and plots of cathodic peak current(Ipa) and anodic peak current(Ipc) vs. scan rate(B)From a to i, the scan rates are 10, 30, 50, 80, 100, 200, 300, 400 and 500 mV/s, respectively.

Fig.5 Cyclic voltammograms of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in PBS with different pH values(A) and plot of cathodic peak current vs. pH(B)pH: a. 5; b. 6; c. 7; d. 8.

Fig.6 Cyclic voltammograms curves of the Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in the absence(a) and presence(b) of 1.0 mmol/L glucose in PBS(pH=7.0) at 100 mV/s

Fig.7 Cyclic voltammograms curves of Dy2(MoO4)3-AuNPs/GOD/CS modified GCE in 0.1 mol/L air-saturated PBS(pH=7.0) with different glucose concentrations(from a to f: 0.01, 0.05, 0.1, 0.3, 0.8, 1.0 mmol/L)(A) and calibration plot of reduction response current vs. glucose concentration(B)

Table 1 Comparison of different modified electrodes

Electrode	Linear range/(mmol·L^-1)	Detection limit/(μmol·L^-1)	Ref.
AuNPs/Al₂O₃/ GOD	1.0—42.5	10	[26]
GR/PAn/Au NPs/GOD	0.2—11.2	100	[27]
PGR-chitosan/GOD	0.14—7.0	17.5	[28]
ERGO-AuNPs/GOD	0.2—2.0	17	[29]
Dy₂(MoO₄)₃-AuNPs/GOD/CS	0.01—1.0	3.33	This work

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基于Dy₂(MoO₄)₃-AuNPs复合纳米材料的葡萄糖生物传感器

Glucose Biosensor Based on Dy₂(MoO₄)₃-AuNPs Composite Nanomaterial^†

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