基于铂纳米颗粒电沉积镁铝水滑石修饰电极的电化学葡萄糖生物传感器

doi:10.7503/cjcu20150881

高等学校化学学报 ›› 2016, Vol. 37 ›› Issue (3): 442.doi: 10.7503/cjcu20150881

基于铂纳米颗粒电沉积镁铝水滑石修饰电极的电化学葡萄糖生物传感器

徐亮, 林有芹, 陈旭(), 路艳罗, 杨文胜

北京化工大学化工资源有效利用国家重点实验室, 北京 100029

收稿日期:2015-11-19 出版日期:2016-03-10 发布日期:2016-01-24
基金资助:
国家自然科学基金(批准号: 201175009)和中央高校基本科研业务费专项资金(批准号: YS1406)资助

Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor^†

XU Liang, LIN Youqin, CHEN Xu^*(), LU Yanluo, YANG Wensheng

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China

Received:2015-11-19 Online:2016-03-10 Published:2016-01-24
Contact: CHEN Xu E-mail:chenxu@mail.buct.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.201175009) and the Fundamental Research Funds for the Central Universities of China(No;YS1406)

摘要/Abstract

摘要：

采用成核/晶化隔离法合成了镁铝水滑石纳米颗粒, 将其修饰到氧化铟锡导电玻璃电极表面; 在此修饰电极基础上, 利用电沉积还原氯铂酸盐法制备了铂纳米颗粒/水滑石复合修饰电极. 由于水滑石层板表面的外限域作用有效抑制了铂纳米颗粒的聚集, 使该电极对过氧化氢具有较好的电催化性能. 基于镁铝水滑石良好的生物相容性, 将葡萄糖氧化酶进一步修饰到该电极表面, 实现了对葡萄糖高灵敏的电化学检测, 检出限(S/N=3)达1.0 μmol/L.

关键词: 铂纳米颗粒, 镁铝水滑石, 电沉积, 电化学葡萄糖生物传感器

Abstract:

MgAl-layered double hydroxide(MgAl-LDH) nanoparticles were synthesized by separate nucleation and aging steps. The MgAl-LDH nanoparticles were then immobilized on the surface of clean indium tin oxide(ITO) electrode. Electrodeposition of platinum nanoparticles(PtNPs) on the MgAl-LDH modified ITO electrode was carried out by electrochemical reduction of PtC $l 6 2 -$ . The obtained PtNPs/MgAl-LDH modified ITO electrodes exhibited high electrocatalysis activity for H₂O₂. After the glucose oxidase(GOD) was further immobilized on the above modified electrode, such prepared electrode was employed as the enzyme electrode for glucose detection. The results showed that the GOD/PtNPs/MgAl-LDH modified ITO electrodes had obviously enhanced the response sensitivity of the glucose biosensor and lowered the detection limit when detecting the H₂O₂ liberated in the enzymatic reaction between GOD and glucose.

Key words: Platinum nanoparticles, MgAl-layered double hydroxide, Electrodeposition, Electrochemical glucose biosensor

中图分类号:

O657.1

TrendMD:

徐亮, 林有芹, 陈旭, 路艳罗, 杨文胜. 基于铂纳米颗粒电沉积镁铝水滑石修饰电极的电化学葡萄糖生物传感器. 高等学校化学学报, 2016, 37(3): 442.

XU Liang, LIN Youqin, CHEN Xu, LU Yanluo, YANG Wensheng. Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor^†. Chem. J. Chinese Universities, 2016, 37(3): 442.

图/表 4

Fig.1 SEM image(A), EDS spectrum(B), XRD pattern(C) and N2-sorption isotherm(D) of the MgAl-LDH nanoparticles

Fig.2 SEM images of PtNPs/MgAl-LDHs/ITO electrode(A), PtNPs/ITO electrode(B) and XRD pattern of PtNPs/MgAl-LDHs/ITO electrode(C) Inset of (A): EDS spectrum of PtNPs/MgAl-LDHs/ITO electrode.

Fig.3 Cyclic voltammograms of PtNPs/MgAl-LDHs/ITO electrode(A), bare ITO(B) and PtNPs/ITO electrode(C) in 0.1 mol/L PBS(pH=7.0) in the absence(a) and presence(b) of 2 mmol/L H2O2 at scan rate of 0.05 V/s and chronoamperometric responses of PtNPs/ITO electrode(a) and PtNPs/MgAl-LDHs/ITO electrode(b) in 0.1 mol/L PBS(pH=7.0) on succession injecting the various concentration H2O2 at working potential of 0.50 V(D)Inset of (D): the magnified part of the curve marked with red square.

Fig.4 Amperometric response of GOD/PtNPs/MgAl-LDHs/ITO electrode in 0.1 mol/L PBS(pH=7.0) on succession injecting the various concentration of glucose at an applied potential of 0.50 V(A) and the corresponding calibration curve of the biosensor for glucose(B)Inset of (A): the magnified part of the curve marked with red square.

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基于铂纳米颗粒电沉积镁铝水滑石修饰电极的电化学葡萄糖生物传感器

Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor^†

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基于铂纳米颗粒电沉积镁铝水滑石修饰电极的电化学葡萄糖生物传感器

Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor†

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Electrodeposition of Platinum Nanoparticles on MgAl-layered Double Hydroxide Modified Indium Tin Oxide Electrode for Electrochemical Glucose Biosensor^†