高等学校化学学报 ›› 1999, Vol. 20 ›› Issue (6): 872.

• 论文 • 上一篇    下一篇

含芘荧光化学敏感器化合物同小牛胸腺DNA分子间的相互作用

李华平, 汪鹏飞, 吴世康   

  1. 中国科学院感光化学研究所, 北京 100101
  • 收稿日期:1998-05-08 出版日期:1999-06-24 发布日期:1999-06-24
  • 通讯作者: 吴世康
  • 作者简介:李华平,男,26岁,硕士.
  • 基金资助:

    国家自然科学基金(批准号:29733100)资助课题

Interaction Between Flourescence Chemosensors with Pyrene and Calf Thymus DNA

LI Hua-Ping, WANG Peng-Fei, WU Shi-Kang   

  1. Institute of Photographic Chemistry, Chinese Academy of Sciences, Beijing, 100101
  • Received:1998-05-08 Online:1999-06-24 Published:1999-06-24

摘要: 研究了含芘荧光化学敏感器分子被ctDNA猝灭的荧光光谱.ctDNA分子对该化学敏感器中芘的激发单体,激基缔合物都有猝灭作用.对激发单体的猝灭速度顺序为;化合物(2)>化合物(1)>芘丁酸>化合物(3);对激基缔合物的猝灭速度顺序为;化合物(2)化合物(3).由得到的荧光猝灭数据,可按公式(2)求得荧光化学敏感器分子与ctDNA分子相互作用的稳定常数.发现化合物(2)与ctDNA分子间有着最强的相互作用能力.按ctDNA和含芘荧光化学敏感器的分子结构、构型以及分子内原子-原子的间距等提出了ctDNA分子与该荧光化学敏感器的作用模型,并对上述结果进行了初步解释.

关键词: 含芘荧光化学敏感器, 小牛胸腺DNA, 分子识别, 荧光猝灭

Abstract: The quenching spectra of fluorescence chemosensors with pyrene by calf thymus DNA were studied in this work. The fluorescence quenching of both monomer and excimer of pyrene by ctDNA were investigated. The quenching constants of fluorescence chemosensors by ctDNA from SternVolmer equation were compared as following: for monomer, compound 2>compound 1>pyrenyl burytic acid>compound 3; for excimer, compound 2compound 3 . The stability constants of the interaction between fluorescence chemosensors and ctDNA were calculated according to equation (2). Of which the interaction between fluorescence chemosensors and ctDNA has been proposed according to their molecular structure, configuration, atom-atom distances and molecular size. The results obtained were discussed preliminarily.

Key words: Fluorescence chemosensor with pyrene, Calf thymus DNA, Molecular recognition, Fluorescence quenching

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