高等学校化学学报 ›› 2019, Vol. 40 ›› Issue (1): 55-61.doi: 10.7503/cjcu20180369

• 分析化学 • 上一篇    下一篇

基于金纳米簇探针的荧光猝灭检测铁离子

刘千榕1,2, 蔡会武1(), 关明2,3, 乔娟2,3, 齐莉2,3()   

  1. 1. 西安科技大学化学与化工学院, 西安 710054
    2. 中国科学院化学研究所活体分析化学院重点实验室, 北京分子科学国家研究中心, 北京 100190
    3. 中国科学院大学, 北京 100049
  • 收稿日期:2018-05-18 出版日期:2019-01-10 发布日期:2018-11-09
  • 作者简介:

    联系人简介: 蔡会武, 男, 博士, 教授, 主要从事纳米材料合成方面的研究. E-mail: caihuiwu@126.com; 齐 莉, 女, 博士, 研究员, 主要从事生化分析应用方面的研究. E-mail: qili@iccas.ac.cn

  • 基金资助:
    国家自然科学基金(批准号: 21727809, 21635008, 21621062)和中国科学院前沿科学重点研究项目(批准号: QYZDJ-SSW-SLH034)资助.

Turn-off Fluorescence of Gold Nanoclusters for Sensing of Ferric Ion

LIU Qianrong1,2, CAI Huiwu1,*(), GUAN Ming2,3, QIAO Juan2,3, QI Li2,3,*()   

  1. 1. School of Chemistry and Chemical Engineering, Xi’an University of Science and Technology, Xi’an 710054, China;
    2. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
    3. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2018-05-18 Online:2019-01-10 Published:2018-11-09
  • Contact: CAI Huiwu,QI Li E-mail:caihuiwu@126.com;qili@iccas.ac.cn
  • Supported by:
    † Supported by the National Natural Science Foundation of China(Nos.21727809, 21635008, 21621062) and the Key Programs of the Chinese Academy of Sciences, China(No.QYZDJ-SSW-SLH034).

摘要:

D-色氨酸为保护剂和还原剂, 采用水热法快速制备了具有强荧光的金纳米簇(D-Trp@AuNCs); 以其作为荧光探针, 建立了基于荧光猝灭的选择性高灵敏检测Fe3+的传感方法. 利用透射电子显微镜(TEM)、 紫外-可见光谱(UV-Vis)和红外光谱(IR)等手段对制备的金纳米簇进行了表征, 并利用荧光光谱研究了D-Trp@AuNCs的荧光性能. 结果表明, D-Trp@AuNCs具有较好的生物相容性, 其最大激发波长为370 nm, 最大发射波长为460 nm; 向金纳米簇溶液中加入Fe3+后, D-Trp@AuNCs的荧光发生明显猝灭, 其猝灭程度与Fe3+的浓度在0.3~500.0 μmol/L范围内呈现良好的线性关系, 检出限为33.1 nmol/L(S/N=3). 将该荧光探针用于实际水样中Fe3+的检测, 回收率为86.6%~106.5%.

关键词: 金纳米簇, D-色氨酸, 荧光猝灭, 铁离子

Abstract:

D-Tryptophan-stabilized gold nanoclusters(D-Trp@AuNCs) were prepared by a simple one-pot synthesis protocol and displayed a specific response to Fe3+. The resultant AuNCs exhibited a strong blue fluorescence with the maximum emission at 440 nm wavelength. Due to the aggregation-induced fluorescence quenching mechanism, appearance of Fe3+ led dramatic fluorescent intensity decrease of AuNCs rather than other cations. Detection limit of the proposed assay for Fe3+ was 33.1 nmol/L, with a good linear range of 0.3—500.0 μmol/L(R2=0.9997). D-Trp@AuNCs were further applied in monitoring Fe3+ in real water samples, providing a new way for fabrication of AuNCs based fluorescent probes and showing great potential in environmental analysis.

Key words: Gold nanoclusters, D-Tryptophan, Fluorescence quenching, Ferric ion

中图分类号: