高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (8): 1306.

• 研究简报 • 上一篇    下一篇

3,3',5,5'-四甲基联苯胺-H2O2-辣根过氧化物酶伏安酶联免疫分析法测定人血清免疫球蛋白E

何亚楠, 陈洪渊   

  1. 南京大学化学化工学院, 南京 210093
  • 收稿日期:1996-06-18 出版日期:1997-08-24 发布日期:1997-08-24
  • 通讯作者: 陈洪渊
  • 作者简介:何亚楠, 男, 36岁, 博士研究生, 副教授.
  • 基金资助:

    国家自然科学基金

3,3',5,5'-Tetramethylbenzidine(TMB)-H2O2-HRP Voltammetric Immunoassay for the Determination of IgE in Human Serum

HE Ya-Nan, CHEN Hong-Yuan   

  1. College of Chemistry and Chemical Engineering, Naming University, Naming 210093
  • Received:1996-06-18 Online:1997-08-24 Published:1997-08-24

PDF (PC)

关键词: 3, 3', 5, 5'-四甲基联苯胺(TMB), 血清兔疫球蛋白E(IgE), ELISA, 示差脉冲伏安法

Abstract: Adifferential pulse voltammetric immunoassay associated with the TMB-H2O2-HRPfor the determination of IgEantibody was developed. The enzymatic reaction product produced by HRPcatalyzing the oxidation of H2O2 with TMBexhibits a sensitive voltammet-ric response at 0.10V(vs.Ag/AgCl) in pH 4.0 phosphate-citrate buffer solution. The de-tection limit of the present method for IgEantibody was 4.0IU/mL, which was four times lower than that obtained by traditional spectrophotometric ELISAprocedure. It has been ap-plied to the determination of IgEin human serum with satisfactory results.

Key words: 3,3',5,5'-Tetramethylbenzidine (TMB), IgE, ELISA, Differential pulse voltammetry

中图分类号: 

TrendMD: