高等学校化学学报

高等学校化学学报 ›› 1997, Vol. 18 ›› Issue (6): 990.

• 论文 • 上一篇    

茯苓菌核多糖的分离和结构分析

张俐娜1, 丁琼1, 张平义1, 冯汉桥2   

  1. 1. 武汉大学化学系, 武汉, 430072;
    2. 中国科学院武汉物理研究所, 武汉, 430072
  • 收稿日期:1996-07-08 出版日期:1997-06-24 发布日期:1997-06-24
  • 通讯作者: 张俐娜, 女, 56岁, 教授.
  • 作者简介:张俐娜, 女, 56岁, 教授.
  • 基金资助:

    国家自然科学基金

Isolation and Structural Analysis of Polysaccharides from the Sclerotium of Poria Cocos Wolf

ZHANG Li-Na1, DING Qiong1, ZHANG Ping-Yi1, FENG Han-Qiao2   

  1. 1. Deparment of Chemistry, Wuhan University, Wuhan, 430072;
    2. Wuhan Institute of Physics, Academia Sinica, Wuhan, 430072
  • Received:1996-07-08 Online:1997-06-24 Published:1997-06-24

PDF (PC)

被引次数

116

摘要： 分别用0.9％NaCl水溶液、热水、0.5mol/LNaOH水溶液和88％甲酸从新鲜茯苓菌核体中提取出5种多糖:PC1、PC2、PC2－A、PC3和PC4.用IR、HPLC、GC、1HNMR和13CNMR等方法分析了它们的组成和结构.结果表明,PC1、PC2和PC2－A由D-葡萄糖、D-木糖、D-甘露糖和D-半乳糖组成,PC1和PC2－A含葡萄糖醛酸,其葡萄糖含量随提取过程的进行逐渐增加.PC3主要由线型β-(1→3)－D-葡聚糖组成,它占茯苓多糖的绝大部分.PC4由β-(1→3)－D-葡聚糖组成,含少量支链和葡萄糖醛酸.

关键词: 茯苓菌核, 多糖, 结构, 核磁共振

Abstract: Five polysaccharides (PC1, PC2, PC2-A, PC3 and PC4) were isolated from a fresh sclerotium of Poria cocos by using 0.9% NaCl aqueous solution, hot water, 0. 5 mol/L NaOHaqueous solution and 88% formic acid, respectively. The components and structures of the polysaccharides were analyzed by IR, GC, HPLC, 13CNMRand 1H NMR. The polysaccharides of PC1, PC2 and PC2-Aconsist of D-glucose, D-xylose, D-mannose and D-galactose, and the D-glucose content increases with the progress of isolation process. The PC1 and PC2-Acontain glucuronic acid. The polysaccharide PC3 contains mainly linearβ-(1-3)-D-glucan, and is the major fraction of Poria cocos polysaccharides. The PC4 is com-posed of β-(1-3)-D-glucan with a few of braching and glucuronic acid.

Key words: Poria Cocos Wolf, Polysaccharide, Structure, 13C NMR

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