高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (7): 1059.

• 研究简报 • 上一篇    下一篇

碱性蛋白质毛细管电泳分离研究

任吉存1, 邓延倬2, 程介克1   

  1. 1. 武汉大学化学系, 武汉 430072;
    2. 武汉大学分析测试科学系, 武汉 430072
  • 收稿日期:1995-06-05 出版日期:1996-07-24 发布日期:1996-07-24
  • 通讯作者: 邓延倬
  • 作者简介:任吉存,男,38岁,博士,副教授,现在湖南师范大学化学系工作.
  • 基金资助:

    国家自然科学基金

Capillary Zone Electrophoresis Separation of Basic Proteins Using Arginine and Lysine as Buffer Additives

REN Ji-Cun1, DENG Yan-Zhuo2, CHENG Jie-Ke1   

  1. 1. Department of Chemistry, Wuhan University, Wuhan 430072;
    2. Department of Analysis and Testing Science, Wuhan University, Wuhan 430072
  • Received:1995-06-05 Online:1996-07-24 Published:1996-07-24

关键词: 毛细管电泳, 碱性蛋白质, 吸附, 精氨酸, 赖氨酸

Abstract: The separation of basic proteins by capillary electrophoresis was plagued by the adsorption of the basic proteins onto the capillary inner surface,Atnethod is presented to solve this problem by using arginine and lysine as buffer additives,It was found that in the neutral or slightly alkaline solution, arginine and lysine can be adsorbed onto the inner surface of capillary to reduce the electroosmotic flow and the adsorption of the proteins efficiently, which results in improvement of resolution efficiency and reproducibility of the migration time and peak height of basic proteins,The experimental results show that arginine and lysine are the efficient buffer additives used in separation of basic proteins.

Key words: Capillary electrophoresis, Basic proteins, Adsorption, Arginine, Lysine

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