高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (4): 528.

• 论文 • 上一篇    下一篇

毛细管电泳柱后化学发光检测装置的研制

黄波, 李建军, 程介克   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1995-06-14 出版日期:1996-04-24 发布日期:1996-04-24
  • 通讯作者: 程介克
  • 作者简介:黄波,女,26岁,博士研究生.
  • 基金资助:

    国家自然科学基金

Construction of End-Column Chemiluminescence Detection System for Capillary Electrophoresis

HUANG Bo, LI Jian-Jun, CHENG Jie-Ke   

  1. Deparment of Chemistry, Wuhan University, Wuhan 430072
  • Received:1995-06-14 Online:1996-04-24 Published:1996-04-24

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被引次数

15

摘要： 报道自行组装的毛细管电泳柱后化学发光检测装置。鲁米诺和N-4-氨基丁基-N-乙基异鲁米诺-H2O2体系用于评价该装置的可行性,得到发光试剂鲁米诺和ABEI较尖锐对称的电泳分离峰,检出限分别为50和290fmol;基于一些金属离子对鲁米带-H2O2的快速动力学催化反应,得到Cr(Ⅲ)和Zn(Ⅱ)离子非常尖锐的分离峰,理论塔板数达1.51×105和1.86×105,检出限为15amol和2fmol.

关键词: 毛细管电泳, 化学发光检测, N-4-氨基丁基-N-乙基异鲁米诺, 铬(Ⅲ), 锌(Ⅱ)

Abstract: Aself-constructed end-column chemiluminescence detection system for capillary electrophoresis has been described.Luminol and N-4-aminobutyl-N-ethylisoluminol(ABEI)H2O2 chemiluminescence systems are used to illustrate the feasibility of this instrument.Comparatively sharp and symmetric electrophoretic peaks are obtained with the detection limit of 50 fmol and 290 fmol for luminol and ABEI, respectively, Cr(Ⅲ) and Zn(Ⅱ) ions are separated with rather sharp peaks due to their fast catalytic effect on the luminol-H2O2 reaction.The theoretical plate number reaches 1.51×105 and 1.86 ×105 for Cr(Ⅲ)and Zn(Ⅱ),the detection limits of which are 15 amol and 2.07 fmol,respectively.

Key words: Capillary electrophoresis, Chemiluminescence detection, N-4-aminobutyl-N-ethylisoluminol(ABEI), Chromium(Ⅲ), Zinc(Ⅱ)

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