高等学校化学学报

高等学校化学学报 ›› 1996, Vol. 17 ›› Issue (3): 460.

• 论文 • 上一篇    下一篇

酶促反应抑制动力学的微量热法研究──氟离子对漆树漆酶催化氧化邻苯二胺的抑制

熊亚, 吴鼎泉, 周光明, 杜予民, 屈松生   

  1. 武汉大学化学系, 武汉 430072
  • 收稿日期:1995-03-08 出版日期:1996-03-24 发布日期:1996-03-24
  • 通讯作者: 熊亚,男,34岁,博士研究生.
  • 作者简介:熊亚,男,34岁,博士研究生.
  • 基金资助:

    国家自然科学基金

The Microcalorimetric Studies on the Inhibition Kinetics of Enzyme-catalyzed Reaction──Fluoride Ion Inhibiting Laccase-catalyzed Oxidation Reaction of o-Phenylenediamine

XIONG Ya, WU Ding-Quan, ZHOU Guang-Ming, DU Yu-Min, QU Song-Sheng   

  1. Department of Chemistry, Wuhan University, Wuhan 430072
  • Received:1995-03-08 Online:1996-03-24 Published:1996-03-24

PDF (PC)

被引次数

4

摘要： 提出一个适合各类可逆性抑制的热动力学方程,并根据各量之间的关系提出了可逆竞争性抑制、非竞争性抑制、反竞争性抑制的热动力学判据以及计算表观米氏常数Km,app和抑制常数Ki等酶促反应生化常数的热动力学公式,并实际应用于NaF对漆树漆酶催化氧化邻苯二胺抑制的微量热法测定。实验初步得出此抑制为非竞争性抑制,求出其Km,app、Ki值。

关键词: 微量热法, 热动力学, 抑制, 漆树漆酶

Abstract: Thermokinetic equation is proposed as Ω0-1=A[S0]-1 + B,which is available for all kinds of reversible inhibitions of single-substrate enzyme-catalyzed reactions.According to the relations between A/B, Band Km,Ωm,the type of the reversible inhibition can be judged as competitive, non-competitive and uncompetitive inhibitions.The thermokinetic formula which can be used to calculated the apparent Michelis constant Km,app,inhibition constant Kand so on are given.The theory proposed has been applied to the kinetic determination of laccase-catalyzed oxidation reaction of o-phenylenediamine inhibited by fluoride ion.The experimental result shows that this reaction belongs to non-competitive inhibition,Km.app=9.712×10-2mol·L-1,Kt=2.090×10-2mol·L-1.

Key words: Microcalorimetry, Thermokinetics, Inhibition, Laccase

TrendMD: